Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Life Sci. 2020 Aug 1;254:117782. doi: 10.1016/j.lfs.2020.117782. Epub 2020 May 12.
This study assessed the prophylactic or therapeutic effects of taurine (TR) and/or hesperidin (HES) on carbon tetrachloride (CCl) induced acute kidney and testicular injury in rats.
Rats were randomly divided into nine experimental groups including control; corn oil; CCl; HES/CCl; TR/CCl; HES + TR/CCl; CCl/HES; CCl/TR; and CCl/HES + TR groups. CCl was intraperitoneally injected with a single dose of 2 ml /kg b.w. HES and TR were orally gavaged twice weekly 100 mg/kg b.w. for four weeks. Kidney function, inflammatory response, sexual hormones, and oxidative stress indicators were assessed. Histomorphological and immune-histochemical studies of the inflammatory marker nuclear factor kappa (NF-κB) in renal and testicular tissues were performed.
The results showed that the TR and/or HES treatment significantly suppressed CCl induced rise of urea, uric acid, potassium, and follicle-stimulating hormone levels. However, significant restoration of sodium, testosterone, and luteinizing hormone was apparent in CCl exposed rats received HES and/or TR. Also, the HES and/or TR treatment significantly rescues CCl induced oxidative stress and inflammation. Moreover, the HES and/or TR dosing significantly repaired the CCl evoked altered renal and testicular architecture and suppressed NF-κB immunoexpression. Notably, alleviating CCl induced renal and testicular damage was more effective in the prophylactic groups than the therapeutic groups. Also, most of the estimated parameters of the HES + TR group did not significantly vary from those of single TR or HES.
In conclusion, HES or TR could efficiently guard against CCl nephro-and reprotoxic effects, but both bioactive combinations afford only a limited synergistic outcome.
本研究评估牛磺酸（TR）和/或橙皮苷（HES）对四氯化碳（CCl）诱导的大鼠急性肾和睾丸损伤的预防或治疗作用。
大鼠随机分为九组实验，包括对照组、玉米油组、CCl 组、HES/CCl 组、TR/CCl 组、HES+TR/CCl 组、CCl/HES 组、CCl/TR 组和 CCl/HES+TR 组。CCl 以 2ml/kg b.w.的剂量腹腔注射，单次给药。HES 和 TR 以 100mg/kg b.w.的剂量每周口服两次，共四周。评估肾功能、炎症反应、性激素和氧化应激指标。对肾脏和睾丸组织中核因子 kappa（NF-κB）炎症标志物进行组织形态学和免疫组织化学研究。
结果表明，TR 和/或 HES 治疗可显著抑制 CCl 诱导的尿素、尿酸、钾和促卵泡激素水平升高。然而，在接受 HES 和/或 TR 治疗的 CCl 暴露大鼠中，钠、睾酮和黄体生成素水平明显恢复。此外，HES 和/或 TR 治疗可显著挽救 CCl 诱导的氧化应激和炎症。此外，HES 和/或 TR 给药可显著修复 CCl 诱发的肾脏和睾丸结构改变，并抑制 NF-κB 免疫表达。值得注意的是，预防组缓解 CCl 诱导的肾和睾丸损伤的效果优于治疗组。此外，HES+TR 组的大多数估计参数与单一 TR 或 HES 相比没有显著差异。
总之，HES 或 TR 可有效防止 CCl 引起的肾和生殖毒性作用，但两种生物活性组合仅提供有限的协同作用。
