B S Aardra, Suresh Vasugi, S Menaka, Sivaperumal Pitchiah
Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Medical Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 Mar 22;16(3):e56680. doi: 10.7759/cureus.56680. eCollection 2024 Mar.
Introduction Marine actinobacteria are promising sources of novel bioactive compounds due to their distinct ecological niches and diverse secondary metabolite production capabilities. Among these, sp. T3S11 is notable for its unique spore chain structure, which allows for both morphological and genetic identification. Despite its potential, little is understood about the secondary metabolites produced by this strain. In this study, we hope to fill this gap by extracting and analyzing the antibacterial activities of secondary metabolites from sp. T3S11, which will be the first time its bioactive compound profile is investigated. Aim To evaluate the antibacterial activity of secondary metabolites isolated from the marine actinobacterium sp. T3S11. Materials and methods The antibacterial assays were carried out on agar plates containing the appropriate media for each pathogen. Sterile filter paper disks were impregnated with secondary metabolites extracted from sp. T3S11 and placed on the surface of agar plates inoculated with the appropriate pathogens. Similarly, disks containing tetracycline were used as a positive control. The plates were then incubated at the appropriate temperature for each pathogen, and the zones of inhibition around the disks were measured to determine the extracted metabolites' antibacterial activity. Result Secondary metabolites had antimicrobial activity against , , and methicillin-resistant (MRSA). The inhibition of was 7.5 mm and 8.5 mm at 75 μg/mL and 100 μg/mL, respectively. zones measured 7 mm and 7.5 mm, while MRSA zones measured 7.6 mm and 8.5 mm at the same concentrations. Tetracycline, the standard antibiotic, had larger inhibition zones: 22 mm for and and 16 mm for MRSA, indicating variable susceptibility. Conclusion We conclude that the secondary metabolites extracted from sp. T3S11 exhibits high antibacterial activity. This could be attributed to the presence of various active compounds.
引言
海洋放线菌由于其独特的生态位和多样的次生代谢产物生产能力，是新型生物活性化合物的有前景来源。其中，[具体菌株名称] sp. T3S11以其独特的孢子链结构而闻名，这有助于进行形态学和遗传学鉴定。尽管具有潜力，但对该菌株产生的次生代谢产物了解甚少。在本研究中，我们希望通过提取和分析来自[具体菌株名称] sp. T3S11的次生代谢产物的抗菌活性来填补这一空白，这将是首次对其生物活性化合物谱进行研究。
目的
评估从海洋放线菌[具体菌株名称] sp. T3S11中分离出的次生代谢产物的抗菌活性。
材料和方法
抗菌试验在含有适合每种病原体的适当培养基的琼脂平板上进行。无菌滤纸圆盘浸渍有从[具体菌株名称] sp. T3S11中提取的次生代谢产物，并放置在接种有适当病原体的琼脂平板表面。同样，含有四环素的圆盘用作阳性对照。然后将平板在适合每种病原体的温度下孵育，并测量圆盘周围的抑菌圈以确定提取的代谢产物的抗菌活性。
结果
次生代谢产物对[具体病原体名称1]、[具体病原体名称2]和耐甲氧西林金黄色葡萄球菌（MRSA）具有抗菌活性。在75μg/mL和100μg/mL时，对[具体病原体名称1]的抑菌圈分别为7.5mm和8.5mm。在相同浓度下，对[具体病原体名称2]的抑菌圈分别为7mm和7.5mm，而对MRSA的抑菌圈分别为7.6mm和8.5mm。标准抗生素四环素具有更大的抑菌圈：对[具体病原体名称1]和[具体病原体名称2]为22mm，对MRSA为16mm，表明敏感性存在差异。
结论
我们得出结论，从[具体菌株名称] sp. T3S11中提取的次生代谢产物具有高抗菌活性。这可能归因于存在各种活性化合物。
