Zeng Weizhu, Shan Xiaoyu, Liu Li, Zhou Jingwen
Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Bioresour Bioprocess. 2022 Nov 26;9(1):121. doi: 10.1186/s40643-022-00610-7.
1,3-Dihydroxyacetone (DHA) is a commercially important chemical and widely used in cosmetics, pharmaceuticals, and food industries as it prevents excessive water evaporation, and provides anti-ultraviolet radiation protection and antioxidant activity. Currently, the industrial production of DHA is based on a biotechnological synthetic route using Gluconobacter oxydans. However, achieving higher production requires more improvements in the synthetic process. In this study, we compared DHA synthesis levels in five industrial wild-type Gluconobacter strains, after which the G. oxydans WSH-003 strain was selected. Then, 16 dehydrogenase genes, unrelated to DHA synthesis, were individually knocked out, with one strain significantly enhancing DHA production, reaching 89.49 g L and 42.27% higher than the wild-type strain. By optimizing the culture media, including seed culture and fermentation media, DHA production was further enhanced. Finally, using an established fed-batch fermentation system, DHA production reached 198.81 g L in a 5 L bioreactor, with a glycerol conversion rate of 82.84%.
1,3 - 二羟基丙酮（DHA）是一种具有重要商业价值的化学品，因其能防止水分过度蒸发，并具有抗紫外线辐射保护和抗氧化活性，而广泛应用于化妆品、制药和食品工业。目前，DHA的工业生产基于使用氧化葡萄糖杆菌的生物技术合成路线。然而，要实现更高的产量，合成过程还需要更多改进。在本研究中，我们比较了五种工业野生型氧化葡萄糖杆菌菌株的DHA合成水平，之后选择了氧化葡萄糖杆菌WSH - 003菌株。然后，分别敲除了16个与DHA合成无关的脱氢酶基因，其中一个菌株的DHA产量显著提高，达到89.49 g/L，比野生型菌株高出42.27%。通过优化培养基，包括种子培养基和发酵培养基，DHA产量进一步提高。最后，使用已建立的分批补料发酵系统，在5 L生物反应器中DHA产量达到198.81 g/L，甘油转化率为82.84%。
