Wang Cong, Chang Dongdong, Zhang Qi, Yu Zhisheng
College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Beijing, 100049, People's Republic of China.
RCEES-IMCAS-UCAS Joint-Lab of Microbial Technology for Environmental Science, Beijing, 100085, People's Republic of China.
Bioresour Bioprocess. 2024 Jan 3;11(1):4. doi: 10.1186/s40643-023-00717-5.
Lignocellulose pretreated using pyrolysis can yield clean energy (such as bioethanol) via microbial fermentation, which can significantly contribute to waste recycling, environmental protection, and energy security. However, the acids, aldehydes, and phenols present in bio-oil with inhibitory effects on microorganisms compromise the downstream utilization and conversion of lignocellulosic pyrolysates. In this study, we constructed a microbial electrolysis cell system for bio-oil detoxification and efficient ethanol production using evolved Escherichia coli to overcome the bioethanol production and utilization challenges highlighted in previous studies. In electrically treated bio-oil media, the E. coli-H strain exhibited significantly higher levoglucosan consumption and ethanol production capacities compared with the control. In undetoxified bio-oil media containing 1.0% (w/v) levoglucosan, E. coli-H produced 0.54 g ethanol/g levoglucosan, reaching 94% of the theoretical yield. Our findings will contribute to developing a practical method for bioethanol production from lignocellulosic substrates, and provide a scientific basis and technical demonstration for its industrialized application.
通过热解预处理的木质纤维素可通过微生物发酵产生清洁能源（如生物乙醇），这对废物回收利用、环境保护和能源安全具有重要意义。然而，生物油中存在的酸、醛和酚类物质对微生物具有抑制作用，这会影响木质纤维素热解产物的下游利用和转化。在本研究中，我们构建了一个微生物电解池系统，用于生物油解毒和高效乙醇生产，利用进化后的大肠杆菌来克服先前研究中突出的生物乙醇生产和利用挑战。在经过电处理的生物油培养基中，与对照相比，大肠杆菌-H菌株表现出显著更高的左旋葡萄糖苷消耗和乙醇生产能力。在含有1.0%（w/v）左旋葡萄糖苷的未解毒生物油培养基中，大肠杆菌-H产生了0.54 g乙醇/g左旋葡萄糖苷，达到理论产量的94%。我们的研究结果将有助于开发一种从木质纤维素底物生产生物乙醇的实用方法，并为其工业化应用提供科学依据和技术示范。
