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骨髓间充质干细胞外泌体联合透明质酸凝胶治疗创伤后骨关节炎的机制研究

Mechanism study of BMSC-exosomes combined with hyaluronic acid gel in the treatment of posttraumatic osteoarthritis.

作者信息

Liu Xianqiang, Chen Yongshuai, Zhang Tao

机构信息

Beichen District Hospital of Traditional Chinese Medicine, China.

出版信息

Heliyon. 2024 Jul 5;10(14):e34192. doi: 10.1016/j.heliyon.2024.e34192. eCollection 2024 Jul 30.

DOI:10.1016/j.heliyon.2024.e34192
PMID:39100446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11295849/
Abstract

OBJECTIVE

To explore the mechanism and efficacy of gel in the treatment of posttraumatic osteoarthritis (PTOA), combined with hyaluronic acid (HA) and bone marrow mesenchymal stem cell exosomes (BMSC-EXOs), and to explain its role in alleviating oxidative stress damage induced by mitochondrial reactive oxygen species (ROS).

METHODS

How is the therapeutic potential of toa influenced by bone marrow mesenchymal stem cells-EXO to be evaluated both in vitro and in vivo. In vitro, BMSC-EXOs were extracted and characterized from rat specimens and labeled with Dil. Rat primary chondrocytes were then isolated to create a cellular PTOA model. BMSC-EXOs + HA group, BMSC-EXOs + HA + 740Y-P group, model group, BMSC-EXOs group, HA group, and control group were included in the cell group, and the function of cartilage matrix and the level of oxidative stress could be evaluated. Cartilage matrix integrity and oxidative stress can be assessed by grouping rats. At the same time, a rat model of ptosis can be established by excision of the anterior cruciate ligament, and joint rehabilitation, with pro-inflammatory and Enzyme-linked immunosorbent assay (ELISA) can be used to determine anti-inflammatory markers.

RESULT

sThe combined use of BMSC-EXOs and HA gel was found to significantly reduce oxidative stress in chondrocytes and PTOA rat models, improving cartilage mechanical properties more effectively than BMSC-EXOs alone.

CONCLUSION

BMSC-EXOs combined with HA gel offer a promising treatment for PTOA by modulating damage caused by mitochondrial ROS-induced oxidative stress.

摘要

目的

探讨凝胶联合透明质酸（HA）和骨髓间充质干细胞外泌体（BMSC-EXOs）治疗创伤后骨关节炎（PTOA）的机制及疗效，并阐述其在减轻线粒体活性氧（ROS）诱导的氧化应激损伤中的作用。

方法

在体外和体内评估骨髓间充质干细胞外泌体对PTOA治疗潜力的影响。体外，从大鼠标本中提取并鉴定BMSC-EXOs，并用Dil标记。然后分离大鼠原代软骨细胞以建立细胞PTOA模型。细胞组包括BMSC-EXOs + HA组、BMSC-EXOs + HA + 740Y-P组、模型组、BMSC-EXOs组、HA组和对照组，可评估软骨基质功能和氧化应激水平。通过对大鼠进行分组可评估软骨基质完整性和氧化应激。同时，通过切除前交叉韧带建立大鼠PTOA模型，并采用促炎和酶联免疫吸附测定（ELISA）来确定抗炎标志物，进行关节康复。

结果

发现BMSC-EXOs与HA凝胶联合使用可显著降低软骨细胞和PTOA大鼠模型中的氧化应激，比单独使用BMSC-EXOs更有效地改善软骨力学性能。

结论

BMSC-EXOs联合HA凝胶通过调节线粒体ROS诱导的氧化应激损伤，为PTOA提供了一种有前景的治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/a393a253c2a2/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/8bed6263bb34/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/ef088895e138/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/e15ce52abee2/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/406dc7526acc/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/d70efde10047/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/25f28b427e4e/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/3213e5a0c73d/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/a393a253c2a2/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/8bed6263bb34/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/ef088895e138/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/e15ce52abee2/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/406dc7526acc/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/d70efde10047/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/25f28b427e4e/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/3213e5a0c73d/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c496/11295849/a393a253c2a2/mmcfigs1.jpg

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