Silva Anelise Fernandes E, Antoniolli Henrique da Rocha Moreira, Zefa Edison, Valente Vera Lúcia da Silva, Deprá Maríndia
Universidade Federal do Rio Grande do Sul, Departamento de Genética, Programa de Pós-Graduação em Genética e Biologia Molecular, Porto Alegre, RS, Brazil.
Universidade Federal de Pelotas, Departamento de Zoologia, Ecologia e Genética, Programa de Pós-Graduação em Biodiversidade Animal, Capão do Leão, RS, Brazil.
Genet Mol Biol. 2024 Apr 15;46(3 Suppl 1):e20230144. doi: 10.1590/1678-4685-GMB-2023-0144. eCollection 2024.
Mitochondrial genomes have provided significant insights into the evolution of several insects. A typical mitogenome contains 37 genes, and variations in gene order can indicate evolutionary relationships between species. In this study, we have assembled the first complete mitogenomes of Endecous chape and E. onthophagus and analyzed the phylogenetic implications for the Gryllidea infraorder. We performed DNA extractions and genome sequencing for both Endecous species. Subsequently, we searched for raw data in the Sequence Read Archive (SRA) in NCBI. Using the SRA data, we assembled the partial mitogenome of Dianemobius nigrofasciatus and annotated the protein-coding genes (PCGs) for nine species. Phylogenomic relationships were reconstructed using Maximum Likelihood (ML) and Bayesian Inference (BI), utilizing the PCGs from 49 Gryllidea species. The mitogenome lengths of E. chape and E. onthophagus are 16,266 bp and 16,023 bp, respectively, while D. nigrofasciatus has a length of 15,359 bp. Our results indicate that species within the infraorder exhibit four types of gene order arrangements that align with their phylogenetic relationships. Both phylogenomic trees displayed strong support, and the ML corroborated with the literature. Gryllidea species have significantly contributed to various fields, and studying their mitogenomes can provide valuable insights into this infraorder evolution.
线粒体基因组为深入了解几种昆虫的进化提供了重要线索。一个典型的线粒体基因组包含37个基因，基因排列顺序的变化可以表明物种之间的进化关系。在本研究中，我们组装了Endecous chape和E. onthophagus的首个完整线粒体基因组，并分析了其对蟋蟀亚目系统发育的影响。我们对这两种Endecous物种进行了DNA提取和基因组测序。随后，我们在NCBI的序列读取存档（SRA）中搜索原始数据。利用SRA数据，我们组装了黑带拟地蟋的部分线粒体基因组，并注释了9个物种的蛋白质编码基因（PCG）。利用49种蟋蟀亚目物种的PCG，通过最大似然法（ML）和贝叶斯推断（BI）重建了系统发育关系。Endecous chape和E. onthophagus的线粒体基因组长度分别为16,266 bp和16,023 bp，而黑带拟地蟋的长度为15,359 bp。我们的结果表明，该亚目内的物种表现出四种与它们的系统发育关系一致的基因排列顺序类型。两个系统发育树都显示出很强的支持度，并且最大似然法的结果与文献一致。蟋蟀亚目物种在各个领域都有显著贡献，研究它们的线粒体基因组可以为该亚目进化提供有价值的见解。
