Bajpai Devika, Rajasekar Arvina
Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 Mar 21;16(3):e56629. doi: 10.7759/cureus.56629. eCollection 2024 Mar.
In the contemporary perspective, periodontitis is considered a complex issue triggered and perpetuated by bacteria but strongly influenced by the way the body reacts to bacterial plaque. Recent research has indicated that variations in genes might have an impact on the development of periodontitis. This study was conducted to explore a probable link between the genetic variations in intercellular adhesion molecule-1 () represented by rs5498 and the occurrence of periodontitis. Methods: A total of 100 participants, 50 with periodontitis and 50 with periodontally healthy or mild gingivitis, were recruited for this study. Whole blood drawn from the participants was used to obtain genomic DNA. The gene polymorphism (rs5498) was determined using polymerase chain reaction (PCR) amplification and digestion. The gene's flanking primers were used to amp up the DNA. For statistical analysis, the genotype that was analyzed using the pattern of restriction fragment length polymorphism was recorded. The Chi-square test compared genotype and allele frequency distributions between both groups. The odds ratio with 95% confidence intervals with each individual allele or genotype was used to compute the risk. Statistical significance was established in all tests when the p-value was less than 0.05.
There was no discernible difference between the genotype frequencies of patients and controls χ2df (P = 0.6065). The findings demonstrated that no significant difference was present between the two groups for homozygous or heterozygous mutant genotypes (AA vs. AG+GG; P = 0.6854). There was no discernible difference in the detected frequencies of the A allele (58% vs. 61%), G allele (42% vs. 39%), TT (16% vs. 24%), AG (40% vs. 36%), and TT genotypes in the studied groups.
According to the results of the current investigation, the (rs5498) gene polymorphism is not associated with periodontitis in the population investigated.
从当代观点来看，牙周炎被认为是一个由细菌引发并持续存在的复杂问题，但身体对牙菌斑的反应方式对其有很大影响。最近的研究表明，基因变异可能会影响牙周炎的发展。本研究旨在探讨以rs5498表示的细胞间黏附分子 -1（）基因变异与牙周炎发生之间可能存在的联系。
本研究共招募了100名参与者，其中50名患有牙周炎，50名牙周健康或患有轻度牙龈炎。采集参与者的全血以获取基因组DNA。使用聚合酶链反应（PCR）扩增和酶切来确定基因多态性（rs5498）。基因的侧翼引物用于扩增DNA。对于统计分析，记录使用限制性片段长度多态性模式分析的基因型。卡方检验比较两组之间的基因型和等位基因频率分布。使用每个单独等位基因或基因型的95%置信区间的优势比来计算风险。当p值小于0.05时，所有测试均具有统计学意义。
患者和对照组的基因型频率之间没有明显差异χ2df（P = 0.6065）。研究结果表明，两组在纯合或杂合突变基因型（AA与AG + GG；P = 0.6854）方面没有显著差异。在研究组中，A等位基因（58%对61%）、G等位基因（42%对39%）、TT（16%对24%）、AG（40%对36%）和TT基因型的检测频率没有明显差异。
根据当前调查结果，在被调查人群中，（rs5498）基因多态性与牙周炎无关。
