Lee Chaeyoung, Park Jung Min, Hillman Prima F, Yoo Minyi, Kim Hye Yeon, Lee Chang-Seok, Nam Sang-Jip
Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea.
Department of Beauty and Cosmetic Science, Eulji University, Seongnam 13135, Republic of Korea.
Biomol Ther (Seoul). 2024 Jul 1;32(4):492-498. doi: 10.4062/biomolther.2023.208. Epub 2024 Apr 23.
Bioassay and HPLC-UV guided fractionations of the crude extract of marine-derived sp. SNA-077 have led to the isolation of a red pigment, undecylprodigiosin . The chemical structure of undecylprodigiosin was revealed by the interpretation of NMR and mass spectroscopic (MS) data. Further, anti-melanogenic effects of undecylprodigiosin were investigated. First, the melanin contents of undecylprodigiosin -treated B16 cells were evaluated. Furthermore, undecylprodigiosin significantly inhibited the key enzymes involved in melanogenesis, including tyrosinase, tyrosinase related protein-1 (TYRP-1), and dopachrome tautomerase (DCT). The mRNA and protein expression levels of Microphthalmia-associated transcriptian factor (MiTF), a critical transcription factor for tyrosinase gene expression, were also suppressed by undecylprodigiosin treatment in B16 analyses. Collectively, our results suggest for the first time that undecylprodigiosin , a potent component isolated from an extract of marine sp. SNA-077, critically exerts the anti-melanogenic ability for melanin synthesis.
对海洋来源的sp. SNA - 077粗提物进行生物测定和高效液相色谱 - 紫外引导分级分离，得到了一种红色色素——十一烷基灵菌红素。通过对核磁共振（NMR）和质谱（MS）数据的解析，揭示了十一烷基灵菌红素的化学结构。此外，还研究了十一烷基灵菌红素的抗黑色素生成作用。首先，评估了用十一烷基灵菌红素处理的B16细胞中的黑色素含量。此外，十一烷基灵菌红素显著抑制了黑色素生成过程中涉及的关键酶，包括酪氨酸酶、酪氨酸酶相关蛋白 - 1（TYRP - 1）和多巴色素互变异构酶（DCT）。在B16分析中，十一烷基灵菌红素处理也抑制了小眼畸形相关转录因子（MiTF）的mRNA和蛋白质表达水平，MiTF是酪氨酸酶基因表达的关键转录因子。总体而言，我们的结果首次表明，从海洋sp. SNA - 077提取物中分离出的有效成分十一烷基灵菌红素对黑色素合成具有关键的抗黑色素生成能力。
