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通过去除紧密结合的钙离子使肌动蛋白硫醇暴露。

Exposure of actin thiols by the removal of tightly held calcium ions.

作者信息

Konno K, Morales M F

出版信息

Proc Natl Acad Sci U S A. 1985 Dec;82(23):7904-8. doi: 10.1073/pnas.82.23.7904.

DOI:10.1073/pnas.82.23.7904
PMID:3865205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC390878/
Abstract

The removal of bound metal ions from G-actin uncovered two thiols, Cys-10 and Cys-257. The uncovering of these thiols requires a free calcium concentration lower than 10 nM. Therefore, participation of one or both thiols in Ca2+ binding is suggested. Actin labeled with N-(5-fluoresceinyl)maleimide in the absence of calcium moves as a doublet in NaDodSO4/PAGE. It is suggested that two conformers are induced by metal removal and labeling.

摘要

从G-肌动蛋白中去除结合的金属离子后发现了两个巯基，即半胱氨酸-10和半胱氨酸-257。这些巯基的暴露需要游离钙浓度低于10 nM。因此，提示一个或两个巯基参与了Ca2+结合。在没有钙的情况下用N-（5-荧光素基）马来酰亚胺标记的肌动蛋白在NaDodSO4/PAGE中以双峰形式移动。提示金属去除和标记诱导了两种构象体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/ea10a2108a7e/pnas00363-0112-h.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/328fc732335d/pnas00363-0111-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/cb177ed054b8/pnas00363-0111-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/6c377eaf52eb/pnas00363-0111-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/084c6d7ade97/pnas00363-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/a77a7cea2c35/pnas00363-0112-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/f25b3f252d99/pnas00363-0112-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/6e9d4fe716e5/pnas00363-0112-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/87124161663f/pnas00363-0112-e.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/db31af6e5daa/pnas00363-0112-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/ea10a2108a7e/pnas00363-0112-h.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/328fc732335d/pnas00363-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/876c7f7dc7b9/pnas00363-0111-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/cb177ed054b8/pnas00363-0111-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/6c377eaf52eb/pnas00363-0111-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/084c6d7ade97/pnas00363-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/a77a7cea2c35/pnas00363-0112-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/f25b3f252d99/pnas00363-0112-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/6e9d4fe716e5/pnas00363-0112-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/87124161663f/pnas00363-0112-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/8bfe7fada595/pnas00363-0112-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/db31af6e5daa/pnas00363-0112-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd82/390878/ea10a2108a7e/pnas00363-0112-h.jpg

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Tissue sulfhydryl groups.组织巯基
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