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真菌介导合成硒-氧化铋-氧化铜多金属纳米颗粒作为一种潜在的替代抗菌剂，用于对抗兽源产超广谱β-内酰胺酶菌。

Fungus-mediated synthesis of Se-BiO-CuO multimetallic nanoparticles as a potential alternative antimicrobial against ESBL-producing of veterinary origin.

作者信息

Rasheed Rida, Uzair Bushra, Raza Abida, Binsuwaidan Reem, Alshammari Nawaf

机构信息

Department of Biological Sciences, International Islamic University, Islamabad, Pakistan.

National Center of Industrial Biotechnology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.

出版信息

Front Cell Infect Microbiol. 2024 Mar 22;14:1301351. doi: 10.3389/fcimb.2024.1301351. eCollection 2024.

DOI:10.3389/fcimb.2024.1301351
PMID:38655284
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11037251/
Abstract

Bacterial infections emerge as a significant contributor to mortality and morbidity worldwide. Emerging extended-spectrum β-lactamase (ESBL) strains provide a greater risk of bacteremia and mortality, are increasingly resistant to antibiotics, and are a major producer of ESBLs. bacteremia-linked mastitis is one of the most common bacterial diseases in animals, which can affect the quality of the milk and damage organ functions. There is an elevated menace of treatment failure and recurrence of bacteremia necessitating the adoption of rigorous alternative treatment approaches. In this study, Se-Boil-CuO multimetallic nanoparticles (MMNPs) were synthesized as an alternate treatment from extract, and their efficiency in treating ESBL was confirmed using standard antimicrobial assays. Scanning electron microscopy, UV-visible spectroscopy, and dynamic light scattering were used to validate and characterize the mycosynthesized Se-BiO-CuO MMNPs. UV-visible spectra of Se-BiO-CuO MMNPs showed absorption peak bands at 570, 376, and 290 nm, respectively. The average diameters of the amorphous-shaped Se-BiO-CuO MMNPs synthesized by extract were approximately 66-80 nm, respectively. Se-BiO-CuO MMNPs (100 μg/mL) showed a maximal inhibition zone of 18.33 ± 0.57 mm against . Se-BiO-CuO MMNPs also exhibited a deleterious impact on killing kinetics, biofilm formation, swimming motility, efflux of cellular components, and membrane integrity. The hemolysis assay also confirms the biocompatibility of Se-BiO-CuO MMNPs at the minimum inhibitory concentration (MIC) range. Our findings suggest that Se-BiO-CuO MMNPs may serve as a potential substitute for ESBL bacteremia.

摘要

细菌感染已成为全球死亡率和发病率的重要因素。新出现的超广谱β-内酰胺酶（ESBL）菌株导致菌血症和死亡的风险更高，对抗生素的耐药性越来越强，并且是ESBLs的主要产生者。与菌血症相关的乳腺炎是动物中最常见的细菌性疾病之一，它会影响牛奶质量并损害器官功能。治疗失败和菌血症复发的威胁不断增加，因此需要采用严格的替代治疗方法。在本研究中，合成了硒-生物炭-氧化铜多金属纳米颗粒（MMNPs）作为提取物的替代治疗方法，并使用标准抗菌试验证实了其治疗ESBL的效果。利用扫描电子显微镜、紫外-可见光谱和动态光散射对真菌合成的硒-生物炭-氧化铜MMNPs进行了验证和表征。硒-生物炭-氧化铜MMNPs的紫外-可见光谱分别在570、376和290nm处显示吸收峰带。由提取物合成的无定形硒-生物炭-氧化铜MMNPs的平均直径分别约为66-80nm。硒-生物炭-氧化铜MMNPs（100μg/mL）对[具体细菌名称未给出]显示出最大抑菌圈为18.33±0.57mm。硒-生物炭-氧化铜MMNPs还对[具体细菌名称未给出]的杀灭动力学、生物膜形成、游动性、细胞成分外流和膜完整性产生有害影响。溶血试验也证实了硒-生物炭-氧化铜MMNPs在最低抑菌浓度（MIC）范围内的生物相容性。我们的研究结果表明，硒-生物炭-氧化铜MMNPs可能作为ESBL菌血症的潜在替代品。

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