Sancer Okan, Şahin Uğur, Çetin Emel Sesli, Tepebaşi Muhammet Yusuf, Cezaroğlu Yasemin, Bilir Göksel, Yünlü Sibel, Koca Ahmet
Suleyman Demirel University, Faculty of Medicine, Department of Medical Biology - Isparta, Turkey.
Suleyman Demirel University, Innovative Technologies Application and Research Center, Genetic Research Unit - Isparta, Turkey.
Rev Assoc Med Bras (1992). 2024 Apr 22;70(3):e20230683. doi: 10.1590/1806-9282.20230683. eCollection 2024.
In this study, we aimed to determine the phenolic compounds, the antibacterial activity of extract from Laurus nobilis leaves, and its possible effect on transforming growth factor-β1 expression level in peripheral blood mononuclear cells.
The phenolic components of Laurus nobilis were identified by the high-performance liquid chromatography method. The antibacterial activity of this extract was determined by disk diffusion and broth microdilution methods. The transforming growth factor-β1 expression was analyzed using the RT-qPCR method.
Epicatechin was found in the highest amount and o-coumaric acid in the lowest amount. The half-maximal inhibitory concentration (IC50) was determined to be 55.17 μg/mL. The zones of inhibition and minimum inhibitory concentration for Staphylococcus aureus, Enterococcus faecalis, and Klebsiella pneumoniae were 15, 14, and 8 mm and 125, 250, and 1000 μg/mL, respectively. The change in transforming growth factor-β1 expression levels was found to be statistically significant compared with the control groups (p<0.0001).
Laurus nobilis extract was found to be effective against bacteria and altered the expression level of transforming growth factor-β1 in peripheral blood mononuclear cells.
在本研究中，我们旨在确定月桂叶提取物中的酚类化合物、抗菌活性及其对外周血单核细胞中转化生长因子-β1表达水平的可能影响。
采用高效液相色谱法鉴定月桂叶中的酚类成分。通过纸片扩散法和肉汤微量稀释法测定该提取物的抗菌活性。使用RT-qPCR法分析转化生长因子-β1的表达。
发现表儿茶素含量最高，邻香豆酸含量最低。半数抑制浓度（IC50）测定为55.17μg/mL。金黄色葡萄球菌、粪肠球菌和肺炎克雷伯菌的抑菌圈直径分别为15、14和8mm，最低抑菌浓度分别为125、250和1000μg/mL。与对照组相比，转化生长因子-β1表达水平的变化具有统计学意义（p<0.0001）。
发现月桂叶提取物对细菌有效，并改变了外周血单核细胞中转化生长因子-β1的表达水平。
