Divilov Konstantin
Department of Fisheries, Wildlife, and Conservation Sciences, Coastal Oregon Marine Experiment Station, Oregon State University, Hatfield Marine Science Center, Newport, Oregon 97365, USA.
Microb Genom. 2024 Apr;10(4). doi: 10.1099/mgen.0.001237.
Molluscan herpesviruses cause disease in species of major importance to aquaculture and are the only known herpesviruses to infect invertebrates, which lack an adaptive immune system. Understanding the evolution of malacoherpesviruses in relation to their hosts will likely require comparative genomic studies on multiple phylogenetic scales. Currently, only two malacoherpesvirus species have genomes that have been fully assembled, which limits the ability to perform comparative genomic studies on this family of viruses. In the present study, we fully assemble a herpesvirus from Illumina and Nanopore sequence data that were previously used to assemble the genome of the gastropod . We tentatively assign this novel herpesvirus to the genus within the family based on a phylogenetic analysis of DNA polymerase. While structurally similar to other malacoherpesvirus genomes, a synteny analysis of the novel herpesvirus with another species indicates that genomic rearrangements might be an important process in the evolution of this genus. We anticipate that future complete assemblies of malacoherpesviruses will be a valuable resource in comparative herpesvirus research.
软体动物疱疹病毒可在对水产养殖至关重要的物种中引发疾病，并且是已知的唯一能感染缺乏适应性免疫系统的无脊椎动物的疱疹病毒。了解软体动物疱疹病毒与其宿主相关的进化过程可能需要在多个系统发育尺度上进行比较基因组研究。目前，只有两种软体动物疱疹病毒的基因组已完全组装完成，这限制了对该病毒家族进行比较基因组研究的能力。在本研究中，我们利用先前用于组装腹足动物基因组的Illumina和Nanopore序列数据，完全组装了一种疱疹病毒。基于DNA聚合酶的系统发育分析，我们初步将这种新型疱疹病毒归为该科内的属。虽然在结构上与其他软体动物疱疹病毒基因组相似，但对该新型疱疹病毒与另一种物种的共线性分析表明，基因组重排可能是该属进化过程中的一个重要过程。我们预计，未来对软体动物疱疹病毒的完整组装将成为比较疱疹病毒研究中的宝贵资源。
