Qinghai Province Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, China.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, China.
Plant Mol Biol. 2024 Apr 24;114(3):50. doi: 10.1007/s11103-024-01445-w.
Amylose biosynthesis is strictly associated with granule-bound starch synthase I (GBSSI) encoded by the Waxy gene. Mutagenesis of single bases in the Waxy gene, which induced by CRISPR/Cas9 genome editing, caused absence of intact GBSSI protein in grain of the edited line. The amylose and amylopectin contents of waxy mutants were zero and 31.73%, while those in the wild type were 33.50% and 39.00%, respectively. The absence of GBSSI protein led to increase in soluble sugar content to 37.30% compared with only 10.0% in the wild type. Sucrose and β-glucan, were 39.16% and 35.40% higher in waxy mutants than in the wild type, respectively. Transcriptome analysis identified differences between the wild type and waxy mutants that could partly explain the reduction in amylose and amylopectin contents and the increase in soluble sugar, sucrose and β-glucan contents. This waxy flour, which showed lower final viscosity and setback, and higher breakdown, could provide more option for food processing.
直链淀粉的合成与粒结合型淀粉合成酶 I（GBSSI）密切相关，该酶由 Waxy 基因编码。通过 CRISPR/Cas9 基因组编辑诱导 Waxy 基因的单个碱基突变，导致编辑系中谷物中完整的 GBSSI 蛋白缺失。蜡质突变体的直链淀粉和支链淀粉含量分别为 0%和 31.73%，而野生型的直链淀粉和支链淀粉含量分别为 33.50%和 39.00%。GBSSI 蛋白的缺失导致可溶糖含量增加到 37.30%，而野生型仅为 10.0%。蜡质突变体中的蔗糖和β-葡聚糖分别比野生型高 39.16%和 35.40%。转录组分析鉴定了野生型和蜡质突变体之间的差异，这些差异部分可以解释直链淀粉和支链淀粉含量的降低以及可溶糖、蔗糖和β-葡聚糖含量的增加。这种蜡质面粉的最终黏度和回生值较低，崩解值较高，可为食品加工提供更多选择。
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