Hiramine C, Hojo K
Tohoku J Exp Med. 1979 Jul;128(3):295-303. doi: 10.1620/tjem.128.295.
Guinea pigs rendered hypersensitive to tuberculin 2 to 3 weeks (early stage) or 10 to 14 weeks (late stage) after sensitization with complete Freund's adjuvant could be completely desensitized by a single or double injections of a sufficient amount of PPD (purified protein derivative). Lymph node cells from such desensitized animals 48 to 72 hr after the challenge showed a considerable reduction of the ability to produce blastogenic factor and skin reactive factor upon PPD-stimulation, whereas the macrophage migration inhibitory factor activity remained still unaffected. As regards antigen-induced 3H-thymidine incorporation in vitro, lymph node cells from animals desensitized in the late stage after sensitization showed no substantial reduction in the degree of enhanced DNA synthesis upon PPD-stimulation, although the desensitization in the early stage resulted in a significant loss of this activity. These results suggest the possibility that desensitizing challenge does not bring about a uniform and regular effect on every lymphocyte subpopulations relevant to different functions and, in addition, indicate that there are some exceptions to the compartmentalization concept of antigen-reactive lymphocytes.
在用完全弗氏佐剂致敏后2至3周（早期）或10至14周（晚期）对结核菌素产生超敏反应的豚鼠，通过单次或两次注射足量的PPD（纯化蛋白衍生物）可被完全脱敏。在激发后48至72小时，来自此类脱敏动物的淋巴结细胞在PPD刺激下产生致有丝分裂因子和皮肤反应因子的能力显著降低，而巨噬细胞迁移抑制因子活性仍未受影响。关于体外抗原诱导的3H - 胸腺嘧啶核苷掺入，致敏后晚期脱敏动物的淋巴结细胞在PPD刺激下DNA合成增强程度没有实质性降低，尽管早期脱敏导致该活性显著丧失。这些结果表明，脱敏激发对与不同功能相关的每个淋巴细胞亚群不会产生统一且规律的效应，此外，还表明抗原反应性淋巴细胞的区室化概念存在一些例外情况。
