Vocational Higher School of Healthcare Studies, Batman University, Main Campus, Health Services Vocational School, Room 217, Kültür Neighborhood, Batman, Turkey.
Department of Geneticy, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, Turkey.
Reprod Sci. 2024 Aug;31(8):2433-2446. doi: 10.1007/s43032-024-01567-y. Epub 2024 Apr 24.
One of the adverse effects of the antineoplastic drug cisplatin (CS) is damage to testicular tissue. This study aimed to examine the potential therapeutic effect of thymoquinone (TQ), a strong antioxidant, against testicular damage caused by CS. In the experiment, 28 rats were used, and the rats were randomly divided into four groups: control (n = 7), CS (n = 7), CS + TQ (n = 7), and TQ (n = 7). The experiment was called off after all treatments were finished on day 15. Blood serum and testicular tissues were utilized for biochemical, histological, immunohistochemical, mRNA expression, and gene protein investigations. The testosterone level decreased and oxidative stress, histopathological damage, dysregulation in mitochondrial dynamics, inflammation and apoptotic cells increased in testicular tissue due to CS administration. TQ supplementation showed anti-inflammatory, antioxidant, and anti-apoptotic effects in response to CS-induced testicular damage. In addition, TQ contributed to the reduction of CS-induced toxic effects by regulating the TNF-α/OTULIN/NF-κB pathway. TQ supplementation may be a potential therapeutic strategy against CS-induced testicular damage by regulating the TNF-α/OTULIN/NF-κB axis, inhibiting inflammation, oxidative stress, and apoptosis.
顺铂（CS）等抗肿瘤药物的一个副作用是对睾丸组织的损伤。本研究旨在探讨强大抗氧化剂胸腺醌（TQ）对 CS 引起的睾丸损伤的潜在治疗作用。实验中使用了 28 只大鼠，将大鼠随机分为四组：对照组（n=7）、CS 组（n=7）、CS+TQ 组（n=7）和 TQ 组（n=7）。所有治疗结束后第 15 天，实验被叫停。血清和睾丸组织用于生化、组织学、免疫组织化学、mRNA 表达和基因蛋白研究。CS 给药导致血清睾酮水平降低，睾丸组织氧化应激、组织病理学损伤、线粒体动力学失调、炎症和凋亡细胞增加。TQ 补充对 CS 诱导的睾丸损伤具有抗炎、抗氧化和抗凋亡作用。此外，TQ 通过调节 TNF-α/OTULIN/NF-κB 通路，有助于减少 CS 引起的毒性作用。TQ 补充可能是通过调节 TNF-α/OTULIN/NF-κB 轴、抑制炎症、氧化应激和凋亡来对抗 CS 诱导的睾丸损伤的一种潜在治疗策略。
