Zhang Xiaoquan, Zhang Yaxuan, Li Man, Jia Hongfang, Wei Fengjie, Xia Zongliang, Zhang Xuelin, Chang Jianbo, Wang Zhaojun
College of Tobacco Science, Henan Agricultural University, Zhengzhou, 450046, China.
Sanmenxia Branch of Henan Provincial Tobacco Corporation, Sanmenxia, 472000, China.
BMC Plant Biol. 2024 Apr 24;24(1):326. doi: 10.1186/s12870-024-04966-0.
Salt stress severely inhibits plant growth, and the WRKY family transcription factors play important roles in salt stress resistance. In this study, we aimed to characterize the role of tobacco (Nicotiana tabacum) NtWRKY65 transcription factor gene in salinity tolerance.
This study characterized the role of tobacco (Nicotiana tabacum) NtWRKY65 transcription factor gene in salinity tolerance using four NtWRKY65 overexpression lines. NtWRKY65 is localized to the nucleus, has transactivation activity, and is upregulated by NaCl treatment. Salinity treatment resulted in the overexpressing transgenic tobacco lines generating significantly longer roots, with larger leaf area, higher fresh weight, and greater chlorophyll content than those of wild type (WT) plants. Moreover, the overexpressing lines showed elevated antioxidant enzyme activity, reduced malondialdehyde content, and leaf electrolyte leakage. In addition, the Na content significantly decreased, and the K/Na ratio was increased in the NtWRKY65 overexpression lines compared to those in the WT. These results suggest that NtWRKY65 overexpression enhances salinity tolerance in transgenic plants. RNA-Seq analysis of the NtWRKY65 overexpressing and WT plants revealed that NtWRKY65 might regulate the expression of genes involved in the salt stress response, including cell wall component metabolism, osmotic stress response, cellular oxidant detoxification, protein phosphorylation, and the auxin signaling pathway. These results were consistent with the morphological and physiological data. These findings indicate that NtWRKY65 overexpression confers enhanced salinity tolerance.
Our results indicated that NtWRKY65 is a critical regulator of salinity tolerance in tobacco plants.
盐胁迫严重抑制植物生长，而WRKY家族转录因子在抗盐胁迫中发挥重要作用。在本研究中，我们旨在阐明烟草（Nicotiana tabacum）NtWRKY65转录因子基因在耐盐性中的作用。
本研究利用四个NtWRKY65过表达株系，对烟草（Nicotiana tabacum）NtWRKY65转录因子基因在耐盐性中的作用进行了表征。NtWRKY65定位于细胞核，具有反式激活活性，并受到NaCl处理的上调。盐处理导致过表达转基因烟草株系的根显著更长，叶面积更大，鲜重更高，叶绿素含量也高于野生型（WT）植株。此外，过表达株系的抗氧化酶活性升高，丙二醛含量降低，叶片电解质渗漏减少。此外，与WT相比，NtWRKY65过表达株系中的Na含量显著降低，K/Na比值增加。这些结果表明，NtWRKY65过表达增强了转基因植物的耐盐性。对NtWRKY65过表达植株和WT植株的RNA-Seq分析表明，NtWRKY65可能调控参与盐胁迫响应的基因表达，包括细胞壁成分代谢、渗透胁迫响应、细胞氧化解毒、蛋白质磷酸化和生长素信号通路。这些结果与形态学和生理学数据一致。这些发现表明，NtWRKY65过表达赋予了增强的耐盐性。
我们的结果表明，NtWRKY65是烟草植株耐盐性的关键调节因子。
