Su Mengxu, Han Zhanjiang, Liu Ying, Liu Meilin, Guo Lu, Wu Jiaju, Wu Xiaofeng
State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Science and Technology, Tarim University, Alar 843300, China.
Int J Mol Sci. 2025 Jun 19;26(12):5869. doi: 10.3390/ijms26125869.
After being subjected to mechanical damage, plants trigger changes in primary and secondary metabolites to enhance their resistance or defenses. However, there are limited studies on the joint use of transcriptomics and metabolomics in investigating leaf damage-related defense mechanisms and their regulation in woody plants. This study investigated the leaf damage defense mechanisms of at the molecular level using transcriptome and secondary metabolome analyses. In total, 4078 differentially expressed genes (DEGs; 1207 up-regulated and 2871 down-regulated) and 30 differential secondary metabolites (DSMs; 21 up-regulated and nine down-regulated) were identified from a transcriptome analysis of controls (CK) and CL-treated leaves after 24 h. Plant-pathogen interactions and the MAPK signaling pathway were important defense pathways that synergized in the early stages of leaf damage in . There were 44 DEGs enriched in the KEGG pathways that encoded 21 WRKY transcription factors. Flavonoid genes were the most abundant. They were mainly enriched in the flavone and flavonol biosynthesis and flavonoid biosynthesis pathways. Sakuranetin and pinocembrin were most frequently associated with the differential metabolites and may be the main flavonoids involved in responding to leaf damage in . This study has far-reaching theoretical and practical significance for understanding the response strategies of to leaf damage and for achieving sustainable management and accurate predictions of artificial forests.
受到机械损伤后，植物会触发初级和次级代谢产物的变化以增强其抗性或防御能力。然而，关于转录组学和代谢组学联合用于研究木本植物叶片损伤相关防御机制及其调控的研究较少。本研究通过转录组和次级代谢组分析，在分子水平上研究了[植物名称未给出]的叶片损伤防御机制。在对对照（CK）和CL处理24小时后的叶片进行转录组分析中，共鉴定出4078个差异表达基因（DEGs；1207个上调和2871个下调）和30种差异次级代谢产物（DSMs；21个上调和9个下调）。植物-病原体相互作用和MAPK信号通路是[植物名称未给出]叶片损伤早期协同作用的重要防御途径。KEGG通路中富集了44个DEGs，它们编码21个WRKY转录因子。类黄酮基因最为丰富。它们主要富集在黄酮和黄酮醇生物合成以及类黄酮生物合成途径中。樱花素和松属素与差异代谢产物关联最为频繁，可能是[植物名称未给出]中参与响应叶片损伤的主要类黄酮。本研究对于理解[植物名称未给出]对叶片损伤的响应策略以及实现人工林的可持续管理和准确预测具有深远的理论和实践意义。
