Department of Oral and Maxillofacial Surgery, University Medical Centre Groningen, Groningen, The Netherlands.
Department of Urology, University Medical Centre Groningen, Groningen, The Netherlands.
BJU Int. 2024 Aug;134(2):268-275. doi: 10.1111/bju.16384. Epub 2024 Apr 24.
To investigate the feasibility of fluorescence molecular imaging (FMI), using cetuximab-800CW, as an intraoperative tool to determine surgical margins in penile squamous cell carcinoma (PSCC).
A total of 11 patients with PSCC received 75 mg cetuximab followed by 15 mg cetuximab-800CW 2 days before surgery. FMI of the whole excision specimen and tissue slices was performed. Fluorescence visualisation was correlated to histopathology. Based on tumour and healthy tissue regions of interest, mean fluorescence intensity was calculated for each individual patient.
Significant differences between tumour and healthy mean fluorescence intensity were found with tumour-to-background ratios of a median (IQR) of 1.51 (0.99) and a mean (SD) of 1.51 (0.32) in the excision specimen and tissue slices, respectively. One patient showed a high relative fluorescence intensity with a signal-to-background ratio of 1.79, corresponding to a tumour-positive margin on fresh frozen sectioning.
In this Phase I study we showed that cetuximab-800CW seems suitable to discriminate PSCC from background tissue. The tracer was well tolerated, and no false positive spots were seen.
探讨使用西妥昔单抗-800CW 进行荧光分子成像（FMI）作为确定阴茎鳞状细胞癌（PSCC）手术切缘的术中工具的可行性。
共 11 例 PSCC 患者在手术前 2 天接受了 75mg 西妥昔单抗和 15mg 西妥昔单抗-800CW。对整个切除标本和组织切片进行 FMI 检查。荧光可视化与组织病理学相关联。基于肿瘤和健康组织的感兴趣区域，计算了每位患者的平均荧光强度。
在切除标本和组织切片中，肿瘤和健康组织的平均荧光强度存在显著差异，肿瘤与背景的比值中位数（IQR）分别为 1.51（0.99）和 1.51（0.32）。1 例患者的相对荧光强度较高，信号与背景的比值为 1.79，对应于新鲜冷冻切片上的肿瘤阳性切缘。
在这项 I 期研究中，我们表明西妥昔单抗-800CW 似乎适合于区分 PSCC 与背景组织。该示踪剂耐受性良好，未见假阳性斑点。
