University Medical Centre Groningen, Department of Nuclear Medicine and Molecular Imaging, Groningen, The Netherlands.
University Medical Centre Groningen, Optical Molecular Imaging Groningen, Groningen, The Netherlands.
J Biomed Opt. 2025 Jan;30(Suppl 1):S13708. doi: 10.1117/1.JBO.30.S1.S13708. Epub 2024 Nov 15.
Shortwave-infrared (SWIR) imaging is reported to yield better contrast in fluorescence-guided surgery than near-infrared (NIR) imaging, due to a reduction in scattering. This benefit of SWIR was shown in animal studies, however not yet in clinical studies with patient samples.
We investigate the potential benefit of SWIR to NIR imaging in clinical samples containing cetuximab-IRDye800CW in fluorescence-guided surgery.
The potential of the epidermal growth factor-targeted NIR dye cetuximab-IRDye800CW in the shortwave range was examined by recording the absorption and emission spectrum. An comparison of NIR and SWIR images using clinical tumor samples of patients with penile squamous cell carcinoma (PSCC) and head and neck squamous cell carcinoma (HNSCC) containing cetuximab-IRDye800CW was performed. The comparison was based on the tumor-to-background ratio and an adapted contrast-to-noise ratio (aCNR) using the standard of care pathology tissue assessment as the golden standard.
Based on the emission spectrum, cetuximab-IRDye800CW can be detected in the SWIR range. In clinical PSCC samples, overall SWIR imaging was found to perform similarly to NIR imaging (NIR imaging is better than SWIR in the 2/7 criteria examined, and SWIR is better than NIR in the 3/7 criteria). However, when inspecting HNSCC data, NIR is better than SWIR in nearly all (5/7) examined criteria. This difference seems to originate from background autofluorescence overwhelming the off-peak SWIR fluorescence signal in HNSCC tissue.
SWIR imaging using the targeted tracer cetuximab-IRDye800CW currently does not provide additional benefit over NIR imaging in clinical samples. Background fluorescence in the SWIR region, resulting in a higher background signal, limits SWIR imaging in HNSCC samples. However, SWIR shows potential in increasing the contrast of tumor borders in PSCC samples, as shown by a higher aCNR over a line.
据报道，与近红外（NIR）成像相比，短波红外（SWIR）成像在荧光引导手术中产生更好的对比度，这是由于散射减少。这种 SWIR 的优势已在动物研究中得到证实，但尚未在含有西妥昔单抗-IR-Dye800CW 的患者样本的临床研究中得到证实。
我们研究 SWIR 对含有西妥昔单抗-IR-Dye800CW 的临床样本中 NIR 成像的潜在益处，以进行荧光引导手术。
通过记录吸收和发射光谱来检查靶向表皮生长因子的近红外染料西妥昔单抗-IR-Dye800CW 在短波范围内的潜力。使用含有西妥昔单抗-IR-Dye800CW 的阴茎鳞状细胞癌（PSCC）和头颈部鳞状细胞癌（HNSCC）患者的临床肿瘤样本比较 NIR 和 SWIR 图像。该比较基于肿瘤与背景的比率和使用标准护理病理组织评估作为金标准的改进对比噪声比（aCNR）。
基于发射光谱，西妥昔单抗-IR-Dye800CW 可在 SWIR 范围内检测到。在临床 PSCC 样本中，总体 SWIR 成像被发现与 NIR 成像表现相似（在检查的 7 个标准中的 2/7 个标准中，NIR 优于 SWIR，而在 3/7 个标准中，SWIR 优于 NIR）。然而，当检查 HNSCC 数据时，NIR 在几乎所有（5/7）检查标准中均优于 SWIR。这种差异似乎源于 HNSCC 组织中的背景自发荧光淹没了 SWIR 荧光信号的非峰值。
在临床样本中，目前使用靶向示踪剂西妥昔单抗-IR-Dye800CW 的 SWIR 成像并未提供比 NIR 成像更多的益处。SWIR 区域中的背景荧光导致更高的背景信号，限制了 HNSCC 样本中的 SWIR 成像。然而，SWIR 显示出在增加 PSCC 样本中肿瘤边界对比度方面的潜力，如通过线的更高 aCNR 所示。
