Johnson P W, Tonzetich J
J Dent Res. 1985 Dec;64(12):1361-4. doi: 10.1177/00220345850640120601.
Type I acid-soluble collagen, suspended in 0.02 M Tris/0.13 M NaCl buffer (pH 7.4), was exposed to air atmosphere admixed with 10.7 X 10(-9) moles of [35S]-labeled dimethyl disulphide/methyl mercaptan mixture in the ratio of 99.82% (CH3S)2/0.18% CH3SH. Gas chromatographic analyses of head-space following one and four days of incubation indicated that all of the CH3SH was absorbed from the head-space by the collagen-containing liquid phase, while the (CH3S)2 concentration of the head-space remained essentially unchanged. Of the total [35S]-activity initially present in the head-space, only 1.0 the total [35S]-activity initially present in the head-space, only 1.0 and 1.86% was absorbed by the collagen-containing liquid phase within one and four days, respectively. Paper chromatographic separation of the reaction mixture showed that only 0.42 and 0.60% of the radioactivity in the head-space was associated with collagen after one and four days of reaction. In comparable [35S]-H2S systems devoid of (CH3S)2, all of the H2S was absorbed within 48 hours of incubation with 18.04% (1 day) and 18.28% (4 days) of the available [35S]-H2S complexed with collagen. The results provide evidence that the reaction of collagen with H2S and CH3SH/(CH3S)2 mixture proceeded via the H2S and CH3SH thiol groups.
将 I 型酸溶性胶原蛋白悬浮于 0.02 M Tris/0.13 M NaCl 缓冲液（pH 7.4）中，置于含有 10.7×10⁻⁹ 摩尔 [³⁵S] 标记的二甲基二硫/甲硫醇混合物的空气氛围中，二者比例为 99.82% (CH₃S)₂/0.18% CH₃SH。孵育 1 天和 4 天后对顶空进行气相色谱分析表明，所有 CH₃SH 均被含胶原蛋白的液相从顶空中吸收，而顶空的 (CH₃S)₂ 浓度基本保持不变。在最初存在于顶空中的总 [³⁵S] 活性中，含胶原蛋白的液相在 1 天和 4 天内分别仅吸收了 1.0% 和 1.86%。反应混合物的纸色谱分离显示，反应 1 天和 4 天后，顶空中仅有 0.42% 和 0.60% 的放射性与胶原蛋白相关。在不含 (CH₃S)₂ 的类似 [³⁵S]-H₂S 体系中，孵育 48 小时内所有 H₂S 均被吸收，18.04%（1 天）和 18.28%（4 天）的可用 [³⁵S]-H₂S 与胶原蛋白络合。结果证明胶原蛋白与 H₂S 和 CH₃SH/(CH₃S)₂ 混合物的反应是通过 H₂S 和 CH₃SH 的硫醇基团进行的。
