溃疡性结肠炎中与N6-甲基腺嘌呤和自噬相关的标记基因的鉴定。
Identification of marker genes associated with N6-methyladenosine and autophagy in ulcerative colitis.
作者信息
Liu Xiao-Yan, Qiao Dan, Zhang Ya-Li, Liu Zi-Xuan, Chen You-Lan, Que Ren-Ye, Cao Hong-Yan, Dai Yan-Cheng
机构信息
Department of Gastroenterology, Shanghai Traditional Chinese Medicine-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China.
Institute of Digestive Diseases, Long Hua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
出版信息
World J Clin Cases. 2024 Apr 6;12(10):1750-1765. doi: 10.12998/wjcc.v12.i10.1750.
BACKGROUND
Both N6-methyladenosine (m6A) methylation and autophagy are considered relevant to the pathogenesis of ulcerative colitis (UC). However, a systematic exploration of the role of the com-bination of m6A methylation and autophagy in UC remains to be performed.
AIM
To elucidate the autophagy-related genes of m6A with a diagnostic value for UC.
METHODS
The correlation between m6A-related genes and autophagy-related genes (ARGs) was analyzed. Finally, gene set enrichment analysis (GSEA) was performed on the characteristic genes. Additionally, the expression levels of four characteristic genes were verified in dextran sulfate sodium (DSS)-induced colitis in mice.
RESULTS
GSEA indicated that BAG3, P4HB and TP53INP2 were involved in the inflammatory response and TNF-α signalling nuclear factor kappa-B. Furthermore, polymerase chain reaction results showed significantly higher mRNA levels of BAG3 and P4HB and lower mRNA levels of FMR1 and TP53INP2 in the DSS group compared to the control group.
CONCLUSION
This study identified four m6A-ARGs that predict the occurrence of UC, thus providing a scientific reference for further studies on the pathogenesis of UC.
背景
N6-甲基腺苷（m6A）甲基化和自噬均被认为与溃疡性结肠炎（UC）的发病机制相关。然而，m6A甲基化与自噬联合作用在UC中的作用仍有待系统探究。
目的
阐明具有UC诊断价值的m6A自噬相关基因。
方法
分析m6A相关基因与自噬相关基因（ARGs）之间的相关性。最后，对特征基因进行基因集富集分析（GSEA）。此外，在葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎中验证了四个特征基因的表达水平。
结果
GSEA表明，BAG3、P4HB和TP53INP2参与炎症反应和TNF-α信号核因子κB。此外，聚合酶链反应结果显示，与对照组相比，DSS组中BAG3和P4HB的mRNA水平显著更高，而FMR1和TP53INP2的mRNA水平更低。
结论
本研究鉴定出四个预测UC发生的m6A-ARGs，从而为进一步研究UC的发病机制提供了科学参考。
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