Microbiology, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, Utrecht 3584 CH, The Netherlands.
Microbiology, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, Utrecht 3584 CH, The Netherlands.
Microbiol Res. 2024 Jul;284:127736. doi: 10.1016/j.micres.2024.127736. Epub 2024 Apr 22.
Blue light is an important signal for fungal development. In the mushroom-forming basidiomycete Schizophyllum commune, blue light is detected by the White Collar complex, which consists of WC-1 and WC-2. Most of our knowledge on this complex is derived from the ascomycete Neurospora crassa, where both WC-1 and WC-2 contain GATA zinc-finger transcription factor domains. In basidiomycetes, WC-1 is truncated and does not contain a transcription factor domain, but both WC-1 and WC-2 are still important for development. We show that dimerization of WC-1 and WC-2 happens independent of light in S. commune, but that induction by light is required for promoter binding by the White Collar complex. Furthermore, the White Collar complex is a promoter of transcription, but binding of the complex alone is not always sufficient to initiate transcription. For its function, the White Collar complex associates directly with the promoters of structural genes involved in mushroom development, like hydrophobins, but also promotes the expression of other transcription factors that play a role in mushroom development.
蓝光是真菌发育的重要信号。在形成蘑菇的担子菌 Schizophyllum commune 中，蓝光由由 White Collar 复合物检测，该复合物由 WC-1 和 WC-2 组成。我们对该复合物的大部分了解都来自于子囊菌 Neurospora crassa，其中 WC-1 和 WC-2 都含有 GATA 锌指转录因子结构域。在担子菌中，WC-1 被截断，不包含转录因子结构域，但 WC-1 和 WC-2 对发育仍然很重要。我们表明，在 S. commune 中，WC-1 和 WC-2 的二聚化独立于光照发生，但 White Collar 复合物与启动子结合需要光照诱导。此外，White Collar 复合物是转录的启动子，但复合物的结合本身并不总是足以启动转录。为了发挥其功能，White Collar 复合物直接与参与蘑菇发育的结构基因的启动子结合，如疏水性蛋白，但也促进了在蘑菇发育中起作用的其他转录因子的表达。
