Asaro Giuseppe A, Solazzo Matteo, Suku Meenakshi, Spurling Dahnan, Genoud Katelyn, Gonzalez Javier Gutierrez, Brien Fergal J O', Nicolosi Valeria, Monaghan Michael G
Department of Mechanical, Manufacturing and Biomedical Engineering, Trinity College Dublin, Dublin, 2 Ireland.
Advanced Materials and BioEngineering Research (AMBER), Centre at Trinity College Dublin and the Royal College of Surgeons in Ireland, Dublin, 2 Ireland.
NPJ 2D Mater Appl. 2023;7(1):44. doi: 10.1038/s41699-023-00409-w. Epub 2023 Jun 27.
Electroconductive biomaterials are gaining significant consideration for regeneration in tissues where electrical functionality is of crucial importance, such as myocardium, neural, musculoskeletal, and bone tissue. In this work, conductive biohybrid platforms were engineered by blending collagen type I and 2D MXene (TiCT) and afterwards covalently crosslinking; to harness the biofunctionality of the protein component and the increased stiffness and enhanced electrical conductivity (matching and even surpassing native tissues) that two-dimensional titanium carbide provides. These MXene platforms were highly biocompatible and resulted in increased proliferation and cell spreading when seeded with fibroblasts. Conversely, they limited bacterial attachment (Staphylococcus aureus) and proliferation. When neonatal rat cardiomyocytes (nrCMs) were cultured on the substrates increased spreading and viability up to day 7 were studied when compared to control collagen substrates. Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) were seeded and stimulated using electric-field generation in a custom-made bioreactor. The combination of an electroconductive substrate with an external electrical field enhanced cell growth, and significantly increased cx43 expression. This in vitro study convincingly demonstrates the potential of this engineered conductive biohybrid platform for cardiac tissue regeneration.
导电生物材料在电功能至关重要的组织（如心肌、神经、肌肉骨骼和骨组织）再生方面正受到广泛关注。在这项工作中，通过将I型胶原蛋白与二维MXene（TiCT）混合，然后进行共价交联，构建了导电生物杂交平台；以利用蛋白质成分的生物功能以及二维碳化钛提供的增加的硬度和增强的电导率（与天然组织匹配甚至超过天然组织）。这些MXene平台具有高度的生物相容性，接种成纤维细胞后可促进细胞增殖和铺展。相反，它们限制了细菌（金黄色葡萄球菌）的附着和增殖。与对照胶原蛋白底物相比，在该底物上培养新生大鼠心肌细胞（nrCMs）时，研究了直至第7天细胞铺展和活力的增加情况。将人诱导多能干细胞衍生的心肌细胞（iPSC-CMs）接种到定制生物反应器中，并利用电场进行刺激。导电底物与外部电场的结合促进了细胞生长，并显著增加了cx43的表达。这项体外研究令人信服地证明了这种工程化导电生物杂交平台在心脏组织再生方面的潜力。
