Liu Chaoqun, Wang Yinquan, Jin Ling, Wang Yan, Liu Dongling
College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, China.
Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co-Constructed by Gansu Province and MOE of PRC, Lanzhou, China.
Front Microbiol. 2024 Apr 11;15:1307966. doi: 10.3389/fmicb.2024.1307966. eCollection 2024.
Lanzhou lily ( var. ) is an exclusive sweet lily variety indigenous to China, which is susceptible to bulbous rot caused by fungal infection during storage. This experiment tests the pathogenicity of the pure culture isolated from the diseased tissue was confirmed in accordance with Koch's postulates, and the pathomycetes were identified based on their morphological and molecular characteristics. Furthermore, the biological characteristics of the pathogens were investigated, followed by an evaluation of the antifungal effects of three plant essential oils against them. The results showed that two strains of fungi were isolated from Lanzhou lily rot, which were identified as Schl. and (Bain. Et sart.). In addition, the pathogenicity of these two strains of fungi was demonstrated that only induced rot with similar symptoms during the post-harvest storage period. The biological characteristics of indicated the potato maltose agar and lily dextrose agar were identified as the most suitable media. Sucrose was determined to be the optimal carbon source, while ammonium nitrate was found to be the best nitrogen source for the growth of . Mycelial growth and sporulation of occurred at an optimum pH value of 6. Total darkness facilitated mycelial growth and conidial germination. The ideal temperature for growth was found to be 28°C, while relative humidity did not significantly impact mycelial growth; however, a relative humidity of 55% was most favorable for spore production. Among the three essential oils tested, cinnamon essential oil displayed superior antifungal efficacy against , whereas angelica essential oil and tea tree essential oil also exhibited moderate inhibitory effects against this pathogen. This research provides valuable theoretical insights for disease control during the storage and transportation of Lanzhou lily.
兰州百合（变种）是中国特有的甜百合品种，在贮藏期间易受真菌感染引起的鳞茎腐烂。本实验对从患病组织中分离得到的纯培养物进行致病性测试，按照科赫法则进行确认，并根据其形态和分子特征对病原菌进行鉴定。此外，还对病原菌的生物学特性进行了研究，随后评估了三种植物精油对它们的抗真菌效果。结果表明，从兰州百合腐烂病中分离出两株真菌，分别鉴定为施氏假单胞菌和（贝恩和萨特）。此外，这两株真菌的致病性表明，在采后贮藏期只有诱导出具有相似症状的腐烂。施氏假单胞菌的生物学特性表明，马铃薯麦芽糖琼脂和百合葡萄糖琼脂被确定为最适合的培养基。蔗糖被确定为最佳碳源，而硝酸铵被发现是施氏假单胞菌生长的最佳氮源。施氏假单胞菌的菌丝生长和孢子形成在最适pH值为6时发生。完全黑暗有利于菌丝生长和分生孢子萌发。发现生长的理想温度为28°C，而相对湿度对菌丝生长没有显著影响；然而，55%的相对湿度最有利于孢子产生。在所测试的三种精油中，肉桂精油对施氏假单胞菌显示出优异的抗真菌效果，而当归精油和茶树精油对该病原菌也表现出中等抑制作用。本研究为兰州百合贮藏和运输期间的病害防治提供了有价值的理论见解。
