Sharif A, Driancourt C, Billard M, Rosenfeld C, Goussault Y, Bourrillon R
Cancer Biochem Biophys. 1985 Dec;8(2):143-52.
An in vitro study of the behaviour of a human acute lymphoblastoid leukemia cell line (REH) towards the action of a mitogenic lectin of Robinia pseudoacacia was carried out. The results were compared with those a reference cell line (LHN13) established from normal human lymphocytes. In both cell lines, the lectin induces agglutination (measured by counting the number of aggregates as well as the number of cells in each aggregate) and decrease of growth (measured by counting the number of cells and the incorporation of tritiated thymidine into TCA-precipitable material per 10(6) cells). The agglutination and the decrease of growth are produced at the doses of 0.5 and 1 microgram/ml of culture medium and after 4 h of exposure of cells to the lectin, respectively. These effects increase progressively with higher doses of lectin and continues throughout the culture. However, the REH line is less sensitive than the LHN13 line to the effects of lectin. Both agglutination and growth decrease of REH as well as LHN13 cell lines by the lectin are reversible; this is confirmed by the fact that the monospecific anti-Robinia lectin serum suppresses these effects.
对人急性淋巴细胞白血病细胞系（REH）针对刺槐有丝分裂原凝集素作用的行为进行了一项体外研究。将结果与从正常人淋巴细胞建立的参考细胞系（LHN13）的结果进行了比较。在这两种细胞系中，凝集素均诱导凝集（通过计数聚集体数量以及每个聚集体中的细胞数量来测量）并抑制生长（通过计数细胞数量以及每10⁶个细胞中氚标记胸腺嘧啶核苷掺入三氯乙酸沉淀物质中的量来测量）。凝集和生长抑制分别在培养基剂量为0.5和1微克/毫升以及细胞暴露于凝集素4小时后产生。这些效应随着凝集素剂量的增加而逐渐增强，并在整个培养过程中持续存在。然而，REH细胞系对凝集素的效应比LHN13细胞系更不敏感。凝集素对REH以及LHN13细胞系的凝集和生长抑制都是可逆的；单特异性抗刺槐凝集素血清抑制这些效应这一事实证实了这一点。
