Vilarem M J, Jouanneau J, Le François D, Bourrillon R
Cancer Res. 1978 Nov;38(11 Pt 1):3960-5.
The comparative aspects of cell growth, i.e., [3H]thymidine and [14C]leucine uptake of low-cancer (P4Bis) and high-cancer (P4BisT) cell lines and of their normal counterparts, have been studied in the presence and absence of concanavalin and Robinia lectins. These lectins have similar effects on cell growth, on thymidine and leucine uptake, and on incorporation of these precursors. The growth of normal cells is stimulated by both lectins, whereas the growth of transformed cells is inhibited. In all cases the uptake of both leucine and thymidine by cells is increased by the lectins, but the percentage of incorporation of the precursors is affected in a different manner. The percentage of thymidine incorporated by normal and transformed cells increases or decreases in direct proportion to cell growth; leucine incorporation is not affected significantly. The reversibility of these lectin effects by specific inhibitors shows that cell membranes are implicated in these phenomena. Our study with normal and transformed cells suggests that cell surface may play a role in the process of malignant transformation and that P4Bis cells are "transitory" between PB1 normal cells and P4BisT high-cancer cells.
在有无伴刀豆球蛋白和刺槐凝集素的情况下，对低癌（P4Bis）和高癌（P4BisT）细胞系及其正常对应细胞的细胞生长的比较方面，即[3H]胸苷和[14C]亮氨酸摄取进行了研究。这些凝集素对细胞生长、胸苷和亮氨酸摄取以及这些前体的掺入具有相似的作用。两种凝集素均刺激正常细胞的生长，而抑制转化细胞的生长。在所有情况下，凝集素均增加细胞对亮氨酸和胸苷的摄取，但前体掺入的百分比受到不同方式的影响。正常细胞和转化细胞掺入胸苷的百分比与细胞生长成正比增加或减少；亮氨酸掺入不受显著影响。这些凝集素作用被特异性抑制剂逆转表明细胞膜与这些现象有关。我们对正常细胞和转化细胞的研究表明，细胞表面可能在恶性转化过程中起作用，并且P4Bis细胞处于PB1正常细胞和P4BisT高癌细胞之间的“过渡”状态。
