Bernard-Griffiths I, Betail G, Godeneche D, Coulet M, Herzog C, Binet J L
Pathol Biol (Paris). 1976 Oct;24(8):517-24.
Some vegetable extract have haemagglutinating activity and are able to transform lymphocytes in culture. The comparative activities of lectin from Pisum sativum and other lectins already used (phytohaere shown. This lectin can be isolated in a highly purified form which is described. Purification of the human blood lymphocytes is obtained through nylon columns and measurement of transformation and proliferation by means of 3 H thymidine incorporation. Normal lymphocyte stimulation can be obtained within a range of concentrations of stimulants can be obtained within a range of concentrations of stimulants and the curves with PHA or lectin from Pisum show a peak of blastogenic activity. The activity of lectin from Pisum sativum was studied in this way on blood cultures of 8 chronic lymphocytic leukaemias and 5 immunodeficient patients. It appeared that lectin from Pisum and PHA have the same activity. The response of human peripheral lymphocytes to various mitogenic agents in vitro does not point out the proliferative abnormal cell and may not be used to detect different populations of human lymphocytes.
一些植物提取物具有血细胞凝集活性，并且能够在培养中转化淋巴细胞。文中展示了来自豌豆的凝集素与其他已使用的凝集素（植物血凝素）的比较活性。这种凝集素可以以高度纯化的形式分离出来并加以描述。通过尼龙柱可实现人血淋巴细胞的纯化，并通过3H胸苷掺入法来测量转化和增殖情况。在一系列刺激剂浓度范围内均可获得正常淋巴细胞刺激，并且来自PHA或豌豆的凝集素的曲线显示出促有丝分裂活性的峰值。通过这种方式，对8例慢性淋巴细胞白血病患者和5例免疫缺陷患者的血液培养物研究了来自豌豆的凝集素的活性。结果表明，来自豌豆的凝集素和PHA具有相同的活性。人外周淋巴细胞在体外对各种促有丝分裂剂的反应并未指出增殖异常细胞，并且可能无法用于检测不同人群的人淋巴细胞。
