Ferheen Ifra, Spurio Roberto, Marcheggiani Stefania
School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy.
Department of Environment and Primary Prevention, National Institute of Health, 00161 Rome, Italy.
Antibiotics (Basel). 2024 Apr 8;13(4):339. doi: 10.3390/antibiotics13040339.
Antibiotic-resistant bacteria (ARB) adhesion onto plastic substrates is a potential threat to environmental and human health. This current research investigates the prevalence of two relevant human pathogens, spp. and spp., and their sophisticated equipment of antibiotic-resistant genes (ARGs), retrieved from plastic substrates submerged into an inland water body. The results of microbiological analysis on selective and chromogenic media revealed the presence of colonies with distinctive phenotypes, which were identified using biochemical and molecular methods. 16S rDNA sequencing and BLAST analysis confirmed the presence of spp., while in the case of spp., 63.6% of strains were found to be members of spp., and the remaining 36.3% were identified as . The Kirby-Bauer disc diffusion assay was performed to test the susceptibility of the isolates to nine commercially available antibiotics, while the genotypic resistant profile was determined for two genes of class 1 integrons and eighteen ARGs belonging to different classes of antibiotics. All isolated bacteria displayed a high prevalence of resistance against all tested antibiotics. These findings provide insights into the emerging risks linked to colonization by potential human opportunistic pathogens on plastic waste commonly found in aquatic ecosystems.
抗生素耐药菌（ARB）在塑料基质上的附着对环境和人类健康构成潜在威胁。当前这项研究调查了两种相关人类病原体——[具体菌种1]和[具体菌种2]的流行情况，以及从内陆水体中浸泡的塑料基质上获取的它们复杂的抗生素耐药基因（ARG）设备。在选择性和显色培养基上进行的微生物分析结果显示存在具有独特表型的菌落，这些菌落通过生化和分子方法进行鉴定。16S rDNA测序和BLAST分析证实了[具体菌种1]的存在，而对于[具体菌种2]，发现63.6%的菌株属于[具体菌种2的一个分类]，其余36.3%被鉴定为[另一个分类]。进行了 Kirby-Bauer 纸片扩散试验以测试分离株对九种市售抗生素的敏感性，同时针对1类整合子的两个基因和属于不同抗生素类别的18个 ARG 确定了基因型耐药谱。所有分离出的细菌对所有测试抗生素都表现出高耐药率。这些发现为水生生态系统中常见的塑料垃圾上潜在人类机会性病原体定殖所带来的新出现风险提供了见解。
