Ferheen Ifra, Spurio Roberto, Marchggiani Stefania
Department of Environment and Primary Prevention, National Institute of Health, 00161 Rome, Italy.
School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy.
STAR Protoc. 2025 Jun 20;6(2):103822. doi: 10.1016/j.xpro.2025.103822. Epub 2025 May 9.
Artificial plastic substrates (APSs) consist of four plastic polymers that work as traps for pathogenic bacteria and facilitate their sampling and detection. Here, we present a protocol for sampling antibiotic-resistant pathogenic bacteria in aquatic ecosystems using APSs. We describe steps for selecting plastic polymers, followed by their assembly, submergence in aquatic environments, and collection for analysis. This protocol can also help explore plastic's role as a new route for disease exposure for humans and animals. For complete details on the use and execution of this protocol, please refer to Ferheen et al..
人工塑料基质（APSs）由四种塑料聚合物组成，这些聚合物可作为致病细菌的捕获器，便于对其进行采样和检测。在此，我们介绍一种使用APSs在水生生态系统中对抗生素抗性致病细菌进行采样的方案。我们描述了选择塑料聚合物的步骤，随后是其组装、浸入水生环境以及收集进行分析。该方案还可帮助探索塑料作为人类和动物疾病暴露新途径的作用。有关该方案使用和执行的完整详细信息，请参考费欣等人的研究。
