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丹麦、芬兰和德国从家禽和人类中分离出的耐超广谱头孢菌素的IncK质粒的比较

Comparison of IncK- Plasmids in Extended-Spectrum Cephalosporin-Resistant Isolated from Poultry and Humans in Denmark, Finland, and Germany.

作者信息

Che Meiyao, Fresno Ana Herrero, Calvo-Fernandez Cristina, Hasman Henrik, Kurittu Paula E, Heikinheimo Annamari, Hansen Lisbeth Truelstrup

机构信息

National Food Institute, Technical University of Denmark, 2800 Lyngby, Denmark.

Department of Biochemistry and Molecular Biology, Faculty of Sciences, Campus Terra, Universidade da Santiago de Compostela (USC), 27002 Lugo, Spain.

出版信息

Antibiotics (Basel). 2024 Apr 10;13(4):349. doi: 10.3390/antibiotics13040349.

DOI:10.3390/antibiotics13040349
PMID:38667025
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11047599/
Abstract

carrying IncK- plasmids mediating resistance to extended-spectrum cephalosporins (ESC) has been frequently described in food-producing animals and in humans. This study aimed to characterize IncK--positive ESC-resistant isolates from poultry production systems in Denmark, Finland, and Germany, as well as from Danish human blood infections, and further compare their plasmids. Whole-genome sequencing (Illumina) of all isolates ( = 46) confirmed the presence of the gene. Minimum inhibitory concentration (MIC) testing revealed a resistant phenotype to cefotaxime as well as resistance to ≥3 antibiotic classes. Conjugative transfer of the gene confirmed the resistance being on mobile plasmids. Pangenome analysis showed only one-third of the genes being in the core with the remainder being in the large accessory gene pool. Single nucleotide polymorphism (SNP) analysis on sequence type (ST) 429 and 1286 isolates showed between 0-60 and 13-90 SNP differences, respectively, indicating vertical transmission of closely related clones in the poultry production, including among Danish, Finnish, and German ST429 isolates. A comparison of 22 ST429 isolates from this study with 80 ST429 isolates in Enterobase revealed the widespread geographical occurrence of related isolates associated with poultry production. Long-read sequencing of a representative subset of isolates ( = 28) allowed further characterization and comparison of the IncK- plasmids with publicly available plasmid sequences. This analysis revealed the presence of highly similar plasmids in ESC-resistant from Denmark, Finland, and Germany pointing to the existence of common sources. Moreover, the analysis presented evidence of global plasmid transmission and evolution. Lastly, our results indicate that IncK- plasmids and their carriers had been circulating in the Danish production chain with an associated risk of spreading to humans, as exemplified by the similarity of the clinical ST429 isolate to poultry isolates. Its persistence may be driven by co-selection since most IncK- plasmids harbor resistance factors to drugs used in veterinary medicine.

摘要

携带介导对超广谱头孢菌素（ESC）耐药的IncK - 质粒的情况在食用动物和人类中已有频繁报道。本研究旨在对来自丹麦、芬兰和德国家禽生产系统以及丹麦人类血液感染中的IncK - 阳性ESC耐药菌株进行特征分析，并进一步比较它们的质粒。对所有分离株（n = 46）进行全基因组测序（Illumina）证实了blaCTX-M基因的存在。最低抑菌浓度（MIC）测试显示对头孢噻肟耐药表型以及对≥3类抗生素耐药。blaCTX-M基因的接合转移证实耐药性存在于可移动质粒上。泛基因组分析表明只有三分之一的基因位于核心区域，其余基因存在于大型辅助基因库中。对序列型（ST）429和1286分离株的单核苷酸多态性（SNP）分析分别显示0 - 60和13 - 90个SNP差异，表明在家禽生产中密切相关克隆的垂直传播，包括丹麦、芬兰和德国的ST429分离株之间。将本研究中的22株ST429分离株与Enterobase中的80株ST429分离株进行比较，发现与家禽生产相关的相关分离株在地理上广泛存在。对一部分代表性分离株（n = 28）进行长读长测序，能够进一步对IncK - 质粒与公开可用的质粒序列进行特征分析和比较。该分析揭示在来自丹麦、芬兰和德国的ESC耐药菌株中存在高度相似的质粒，表明存在共同来源。此外，该分析提供了全球质粒传播和进化的证据。最后，我们的结果表明IncK - 质粒及其携带菌株在丹麦生产链中传播，存在传播给人类的相关风险，临床ST429分离株与家禽分离株的相似性就是例证。其持续存在可能是由共选择驱动的，因为大多数IncK - 质粒携带对兽用药物的耐药因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/8e5f472047ff/antibiotics-13-00349-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/ccbf2d518bf5/antibiotics-13-00349-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/d53ba083ca62/antibiotics-13-00349-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/711ca076bcc3/antibiotics-13-00349-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/7a52e379915c/antibiotics-13-00349-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/7d4ad8ed736b/antibiotics-13-00349-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/8e5f472047ff/antibiotics-13-00349-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/ccbf2d518bf5/antibiotics-13-00349-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/d53ba083ca62/antibiotics-13-00349-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/711ca076bcc3/antibiotics-13-00349-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/7a52e379915c/antibiotics-13-00349-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/7d4ad8ed736b/antibiotics-13-00349-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40cd/11047599/8e5f472047ff/antibiotics-13-00349-g006.jpg

相似文献

[1]
Comparison of IncK- Plasmids in Extended-Spectrum Cephalosporin-Resistant Isolated from Poultry and Humans in Denmark, Finland, and Germany.

Antibiotics (Basel). 2024-4-10

[2]
Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/bla resistance plasmids.

Clin Microbiol Infect. 2017-1-7

[3]
Plasmid and Host Strain Characteristics of Escherichia coli Resistant to Extended-Spectrum Cephalosporins in the Norwegian Broiler Production.

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[4]
Transfer Potential of Plasmids Conferring Extended-Spectrum-Cephalosporin Resistance in Escherichia coli from Poultry.

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[5]
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Appl Environ Microbiol. 2016-7-15

[6]
Prevalence and Transmission of Extended-Spectrum Cephalosporin (ESC) Resistance Genes in Isolated from Poultry Production Systems and Slaughterhouses in Denmark.

Antibiotics (Basel). 2023-11-8

[7]
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[8]
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[9]
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[10]
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本文引用的文献

[1]
Prevalence and Transmission of Extended-Spectrum Cephalosporin (ESC) Resistance Genes in Isolated from Poultry Production Systems and Slaughterhouses in Denmark.

Antibiotics (Basel). 2023-11-8

[2]
Antibiotic Resistance Profiles and Molecular Characteristics of -Carrying Serovar Albany Isolated from Chickens During 2013-2020 in South Korea.

Foodborne Pathog Dis. 2023-11

[3]
Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying in recovered from migrating barnacle geese ( ).

Open Res Eur. 2021-5-4

[4]
Genetic characterization of ESBL-producing and ciprofloxacin-resistant from Belgian broilers and pigs.

Front Microbiol. 2023-4-6

[5]
Prevalence of Potential Pathogenic and Antimicrobial Resistant in Danish Broilers.

Antibiotics (Basel). 2023-2-7

[6]
Distinct evolutionary trajectories in the pangenome occur within sequence types.

Microb Genom. 2022-11

[7]
Comparative genetic characterization of CMY-2-type beta-lactamase producing pathogenic Escherichia coli isolated from humans and pigs suffering from diarrhea in Korea.

Ann Clin Microbiol Antimicrob. 2023-1-19

[8]
Genomic diversity, pathogenicity and antimicrobial resistance of Escherichia coli isolated from poultry in the southern United States.

BMC Microbiol. 2023-1-16

[9]
Dynamics of extended-spectrum cephalosporin resistance genes in Escherichia coli from Europe and North America.

Nat Commun. 2022-12-12

[10]
Detection of Extended-Spectrum β-Lactamases (ESBLs) and AmpC in Class A and Class B Carbapenemase-Producing .

Microbiol Spectr. 2022-12-21

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