Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si, Republic of Korea.
Foodborne Pathog Dis. 2023 Nov;20(11):492-501. doi: 10.1089/fpd.2023.0034. Epub 2023 Sep 12.
The production of β-lactamase by nontyphoidal has become a public health issue throughout the world. In this study, we aimed to investigate the antimicrobial resistance profiles and molecular characteristics of β-lactamase-producing serovar Albany isolates. A total of 434 Albany were obtained from feces and carcasses of healthy and diseased food-producing animals [cattle ( = 2), pigs ( = 3), chickens ( = 391), and ducks ( = 38)] during 2013-2020. Among the 434 Albany isolates, 3.7% showed resistance to cefoxitin, and all the cefoxitin-resistant isolates were obtained from chickens. Moreover, Albany isolates demonstrated high resistance to nalidixic acid (99.3%), trimethoprim/sulfamethoxazole (97.9%), ampicillin (86.6%), chloramphenicol (86.6%), and tetracycline (85.7%), as well as higher rates of multidrug resistance were detected in cefoxitin-resistant isolates compared to cefoxitin-susceptible isolates. All cefoxitin-resistant isolates harbored CMY-2-type β-lactamase and belonged to seven different pulsotypes, with type IV-b (43.75%) and IV-a (25%) making up the majority. In addition, genes encoding cefoxitin resistant of all -harboring Albany isolates were horizontally transmitted to a recipient J53 by conjugation. Furthermore, 93.75% (15/16) of conjugative plasmids harboring genes belong to ST12/CC12-IncI1. Genetic characteristics of transmitted genes were associated with IS, which can play an essential role in the effective mobilization and expression of these genes. Albany containing in chickens can potentially be transferred to humans. Therefore, it is necessary to restrict antibiotic use and conduct continuous monitoring and analysis of resistant bacteria in the poultry industry.
产β-内酰胺酶非伤寒 已成为全世界的一个公共卫生问题。在这项研究中，我们旨在研究产β-内酰胺酶 血清型奥尔巴尼分离株的抗菌药物耐药谱和分子特征。2013 年至 2020 年期间，从健康和患病的食用动物（牛[=2]、猪[=3]、鸡[=391]和鸭[=38]）的粪便和尸体中获得了 434 株 奥尔巴尼。在 434 株 奥尔巴尼分离株中，有 3.7%对头孢西丁表现出耐药性，所有头孢西丁耐药株均来自鸡。此外， 奥尔巴尼分离株对萘啶酸（99.3%）、甲氧苄啶/磺胺甲恶唑（97.9%）、氨苄西林（86.6%）、氯霉素（86.6%）和四环素（85.7%）表现出高度耐药，并且头孢西丁耐药株比头孢西丁敏感株检测到更高的多药耐药率。所有头孢西丁耐药株均携带 CMY-2 型β-内酰胺酶，属于 7 种不同的脉冲型，其中 IV-b（43.75%）和 IV-a（25%）占多数。此外，所有携带 基因的头孢西丁耐药 奥尔巴尼分离株的头孢西丁耐药基因通过接合转移到受体 J53。此外，携带 基因的 93.75%（15/16）的接合质粒属于 ST12/CC12-IncI1。携带 基因的传递质粒的遗传特征与 IS 相关，这在这些基因的有效动员和表达中起着重要作用。鸡中携带 的 奥尔巴尼可能会转移给人类。因此，有必要限制抗生素的使用，并对家禽业中的耐药菌进行持续监测和分析。
