Razooqi Zeinab, Höglund Åberg Carola, Kwamin Francis, Claesson Rolf, Haubek Dorte, Oscarsson Jan, Johansson Anders
Department of Odontology, Umeå University, 901 87 Umeå, Sweden.
Dental School University of Ghana, Korle-Bu, Accra KB 460, Ghana.
Microorganisms. 2022 Dec 19;10(12):2511. doi: 10.3390/microorganisms10122511.
The aims of the present study were to document the presence of and the emerging oral pathogen , as well as to identify genotypes of these bacterial species with enhanced virulence. In addition, these data were analyzed in relation to periodontal pocket depth (PPD) and the progression of PPD from the sampled periodontal sites during a two-year period. Subgingival plaque samples were collected from 172 periodontal pockets of 68 Ghanaian adolescents. PPD at sampling varied from 3-14 mm and the progression from baseline, i.e., two years earlier up to 8 mm. The levels of and were determined with quantitative PCR. The highly leukotoxic JP2-genotype of and the a gene of , encoding a putative Repeats-in-Toxin (RTX) protein, were detected with conventional PCR. The prevalence of was 57%, and 14% of the samples contained the JP2 genotype. was detected in 92% of the samples and the gene in 52%. The levels of these bacterial species were significantly associated with enhanced PPD and progression, with a more pronounced impact in sites positive for the JP2 genotype or the gene. Taken together, the results indicate that the presence of both and with their RTX proteins are linked to increased PPD and progression of disease.
本研究的目的是记录新兴口腔病原体的存在情况，以及鉴定这些具有增强毒力的细菌种类的基因型。此外，还结合牙周袋深度（PPD）以及在两年期间从采样牙周部位的PPD进展情况对这些数据进行了分析。从68名加纳青少年的172个牙周袋中采集了龈下菌斑样本。采样时的PPD为3 - 14毫米，从基线（即两年前）到当时的进展为8毫米。采用定量PCR测定和的水平。用常规PCR检测的高白细胞毒性JP2基因型和编码假定毒素重复序列（RTX）蛋白的基因。的患病率为57%，14%的样本含有JP2基因型。92%的样本检测到，52%的样本检测到基因。这些细菌种类的水平与PPD增加和进展显著相关，在JP2基因型或基因阳性的部位影响更为明显。综上所述，结果表明和及其RTX蛋白的存在与PPD增加和疾病进展有关。
