Németh Krisztián, Sterczer Ágnes, Kiss Dávid Sándor, Lányi Réka Katalin, Hemző Vivien, Vámos Kriszta, Bartha Tibor, Buzás Anna, Lányi Katalin
Department of Physiology and Biochemistry, University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary.
Department of Internal Medicine, University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary.
Metabolites. 2024 Mar 22;14(4):178. doi: 10.3390/metabo14040178.
The comprehensive examination of bile acids is of paramount importance across various fields of health sciences, influencing physiology, microbiology, internal medicine, and pharmacology. While enzymatic reaction-based photometric methods remain fundamental for total BA measurements, there is a burgeoning demand for more sophisticated techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) for comprehensive BA profiling. This evolution reflects a need for nuanced diagnostic assessments in clinical practice. In canines, a BA assessment involves considering factors, such as food composition, transit times, and breed-specific variations. Multiple matrices, including blood, feces, urine, liver tissue, and gallbladder bile, offer insights into BA profiles, yet interpretations remain complex, particularly in fecal analysis due to sampling challenges and breed-specific differences. Despite ongoing efforts, a consensus regarding optimal matrices and diagnostic thresholds remains elusive, highlighting the need for further research. Emphasizing the scarcity of systematic animal studies and underscoring the importance of ap-propriate sampling methodologies, our review advocates for targeted investigations into BA alterations in canine pathology, promising insights into pathomechanisms, early disease detection, and therapeutic avenues.
胆汁酸的综合检测在健康科学的各个领域都至关重要，对生理学、微生物学、内科医学和药理学均有影响。虽然基于酶促反应的光度法仍是总胆汁酸测量的基础方法，但对于更复杂的技术（如液相色谱 - 串联质谱法（LC-MS/MS））以进行胆汁酸综合分析的需求正在迅速增长。这种发展反映了临床实践中对细致入微的诊断评估的需求。在犬类中，胆汁酸评估涉及考虑食物成分、转运时间和品种特异性差异等因素。多种基质，包括血液、粪便、尿液、肝脏组织和胆囊胆汁，都能提供有关胆汁酸谱的信息，但解读仍然复杂，特别是在粪便分析中，由于采样挑战和品种特异性差异。尽管一直在努力，但关于最佳基质和诊断阈值的共识仍然难以达成，这凸显了进一步研究的必要性。我们的综述强调了系统性动物研究的稀缺性，并强调了适当采样方法的重要性，主张针对犬类病理学中胆汁酸变化进行有针对性的研究，有望深入了解发病机制、早期疾病检测和治疗途径。
