Comito Rossana, Porru Emanuele, Interino Nicolò, Conti Matteo, Terragni Rossella, Gotti Roberto, Candela Marco, Simoni Patrizia, Roda Aldo, Fiori Jessica
Department of Medical and Surgical Sciences, Alma Mater Studiorum-University of Bologna, 40138 Bologna, Italy.
Biostructures and Biosystems National Institute (INBB), 00136 Rome, Italy.
Metabolites. 2023 Aug 30;13(9):980. doi: 10.3390/metabo13090980.
Bile acids (BAs), endogenous acidic steroids synthetized from cholesterol in the liver, play a key role in the gut-liver axis physiopathology, including in hepatotoxicity, intestinal inflammatory processes, and cholesterol homeostasis. Faecal Oxo-BAs, relatively stable intermediates of oxidation/epimerization reactions of the BA hydroxyls, could be relevant to investigating the crosstalk in the liver-gut axis and the relationship between diseases and alterations in microbiota composition. A paucity of information currently exists on faecal BA profiles in dogs with and without chronic inflammatory enteropathy (CIE). Comprehensive assessment of 31 molecules among faecal BAs and related microbiota metabolites was conducted with high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Odds ratios (ORs) for associations of BAs with CIE were estimated using logistic regression. Principal component analysis was performed to find differences between the control and pathological dogs. Higher levels of primary BAs and muricholic acids, and lower levels of secondary BAs were found in pathological dogs. Higher concentrations in faecal oxo-metabolites were associated with the absence of CIE (OR < 1). This study shows a marked difference in faecal BA profiles between dogs with and without CIE. Further research will be needed to better understand the role of oxo-BAs and muricholic acids in CIE dogs.
胆汁酸（BAs）是肝脏中由胆固醇合成的内源性酸性甾体，在肠-肝轴生理病理学中起关键作用，包括肝毒性、肠道炎症过程和胆固醇稳态。粪便中的氧代胆汁酸（Oxo-BAs）是胆汁酸羟基氧化/差向异构化反应相对稳定的中间体，可能与研究肝-肠轴中的相互作用以及疾病与微生物群组成改变之间的关系有关。目前关于患有和未患有慢性炎症性肠病（CIE）的犬类粪便胆汁酸谱的信息匮乏。使用高效液相色谱串联质谱法（HPLC-MS/MS）对粪便胆汁酸和相关微生物群代谢产物中的31种分子进行了综合评估。使用逻辑回归估计胆汁酸与CIE关联的比值比（OR）。进行主成分分析以找出对照犬和患病犬之间的差异。在患病犬中发现初级胆汁酸和鼠胆酸水平较高，而次级胆汁酸水平较低。粪便氧代代谢产物中较高的浓度与无CIE相关（OR<1）。本研究表明，患有和未患有CIE的犬类粪便胆汁酸谱存在显著差异。需要进一步研究以更好地了解氧代胆汁酸和鼠胆酸在患有CIE的犬类中的作用。
