• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

[具体植物名称]及其根提取物对[具体植物名称]代谢组的影响 。 需注意，你提供的原文中存在部分信息缺失，我按照格式进行了大致补充完整以便于理解，实际翻译时你应根据准确完整的原文进行。

Influence of and Root Extracts on Metabolome of .

作者信息

Maximo Héros José, Araújo Francisca Diana da Silva, Pagotto Carolina Clepf, Boava Leonardo Pires, Dalio Ronaldo José Durigan, Duarte Gustavo Henrique Bueno, Eberlin Marcos Nogueira, Machado Marcos Antonio

机构信息

Biotechnology Laboratory, Centro de Citricultura Sylvio Moreira, Agronomic Institute, Cordeirópolis 13490-970, SP, Brazil.

BioXyz Biotecnologia Microbiana e Bioprocessos e Industriais Ltda., Piracicaba 13414-224, SP, Brazil.

出版信息

Metabolites. 2024 Apr 5;14(4):206. doi: 10.3390/metabo14040206.

DOI:10.3390/metabo14040206
PMID:38668334
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11052222/
Abstract

is an oomycete pathogen that infects a broad range of crops of worldwide economic interest; among them are citrus species. In general, some and the rootstocks of related genera offer considerable resistance against ; therefore, understanding the mechanisms involved in the virulence of this pathogen is crucial. In this work, secondary metabolite production was studied using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) and ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC/ESI-Q-TOF-MS) combined with chemometric tools, and its metabolic profile was evaluated under the influence of (a highly susceptible host) and (a resistant genotype) extracts. The root extracts of had an influence on the growth and hyphae morphology, and the root extracts of had an influence on the zoospore behavior. In parallel, the spatial distribution of several metabolites was revealed in colonies using MALDI-MSI, and the metabolite ion of 246 was identified as the protonated molecule of Arg-Ala. The MALDI-MSI showed variations in the surface metabolite profile of under the influence of the extract. The metabolome analysis using UHPLC-ESI-Q-TOF-MS resulted in the detection of Arg-Gln (/ 303.1775), as well as L-arginine (/ 175.1191) and other unidentified metabolites. Significant variations in this metabolome were detected under the influence of the plant extracts when evaluated using UHPLC-ESI-Q-TOF-MS. Both techniques proved to be complementary, offering valuable insights at the molecular level when used to assess the impact of the plant extracts on microbial physiology in vitro. The metabolites identified in this study may play significant roles in the interaction or virulence of , but their functional characterization remains to be analyzed. Overall, these data confirm our initial hypotheses, demonstrating that has the capabilities of (i) recognizing host signals and altering its reproductive programing and (ii) distinguishing between hosts with varying responses in terms of reproduction and the production of secondary metabolites.

摘要

是一种卵菌病原体，可感染全球范围内具有经济价值的多种作物；其中包括柑橘类物种。一般来说，一些相关属的砧木对具有相当的抗性；因此，了解这种病原体毒力的相关机制至关重要。在这项工作中，使用基质辅助激光解吸/电离质谱成像（MALDI-MSI）和超高效液相色谱与电喷雾电离四极杆飞行时间串联质谱（UHPLC/ESI-Q-TOF-MS）结合化学计量工具研究了次级代谢产物的产生，并在（一种高度敏感的宿主）和（一种抗性基因型）提取物的影响下评估了其代谢谱。的根提取物对生长和菌丝形态有影响，的根提取物对游动孢子行为有影响。同时，使用MALDI-MSI揭示了菌落中几种代谢物的空间分布，并且将246的代谢物离子鉴定为Arg-Ala的质子化分子。MALDI-MSI显示在提取物的影响下表面代谢物谱的变化。使用UHPLC-ESI-Q-TOF-MS进行的代谢组分析导致检测到Arg-Gln（/ 303.1775），以及L-精氨酸（/ 175.1191）和其他未鉴定的代谢物。当使用UHPLC-ESI-Q-TOF-MS评估时，在植物提取物的影响下检测到该代谢组的显著变化。这两种技术被证明是互补的，当用于评估植物提取物对体外微生物生理学的影响时，在分子水平上提供了有价值的见解。本研究中鉴定的代谢物可能在的相互作用或毒力中起重要作用，但其功能表征仍有待分析。总体而言，这些数据证实了我们最初的假设，表明具有（i）识别宿主信号并改变其繁殖程序以及（ii）区分在繁殖和次级代谢产物产生方面具有不同反应的宿主的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/0658a4a60ef2/metabolites-14-00206-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/2a412d916416/metabolites-14-00206-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/99192d3e0f0d/metabolites-14-00206-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/d9dba8bccc0f/metabolites-14-00206-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/ba4f1ff57230/metabolites-14-00206-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/89f1d22bac59/metabolites-14-00206-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/1d92bf82ccb9/metabolites-14-00206-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/1d7d3346d18e/metabolites-14-00206-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/0658a4a60ef2/metabolites-14-00206-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/2a412d916416/metabolites-14-00206-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/99192d3e0f0d/metabolites-14-00206-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/d9dba8bccc0f/metabolites-14-00206-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/ba4f1ff57230/metabolites-14-00206-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/89f1d22bac59/metabolites-14-00206-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/1d92bf82ccb9/metabolites-14-00206-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/1d7d3346d18e/metabolites-14-00206-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa73/11052222/0658a4a60ef2/metabolites-14-00206-g008.jpg

相似文献

1
Influence of and Root Extracts on Metabolome of .[具体植物名称]及其根提取物对[具体植物名称]代谢组的影响 。 需注意，你提供的原文中存在部分信息缺失，我按照格式进行了大致补充完整以便于理解，实际翻译时你应根据准确完整的原文进行。
Metabolites. 2024 Apr 5;14(4):206. doi: 10.3390/metabo14040206.
2
Molecular Basis of Citrus sunki Susceptibility and Poncirus trifoliata Resistance Upon Phytophthora parasitica Attack.在疫霉菌攻击下，柑橘潜叶蛾易感性和枳抵抗性的分子基础。
Mol Plant Microbe Interact. 2018 Mar;31(3):386-398. doi: 10.1094/MPMI-05-17-0112-FI. Epub 2018 Jan 30.
3
Global gene expression of Poncirus trifoliata, Citrus sunki and their hybrids under infection of Phytophthora parasitica.枳属、甜橙及其杂种感染疫霉菌后的全球基因表达。
BMC Genomics. 2011 Jan 17;12:39. doi: 10.1186/1471-2164-12-39.
4
PpCRN7 and PpCRN20 of Phythophthora parasitica regulate plant cell death leading to enhancement of host susceptibility.寄生疫霉 PpCRN7 和 PpCRN20 调控植物细胞死亡，导致宿主易感性增强。
BMC Plant Biol. 2019 Dec 6;19(1):544. doi: 10.1186/s12870-019-2129-8.
5
QTLs and eQTLs mapping related to citrandarins' resistance to citrus gummosis disease.与枸椽酸抵抗柑橘流胶病相关的 QTLs 和 eQTLs 定位。
BMC Genomics. 2018 Jul 3;19(1):516. doi: 10.1186/s12864-018-4888-2.
6
Identification of QTLs associated with citrus resistance to Phytophthora gummosis.与柑橘对疫霉流胶病抗性相关的数量性状位点的鉴定。
J Appl Genet. 2006;47(1):23-8. doi: 10.1007/BF03194595.
7
Resistance to Phytophthora citrophthora and P. parasitica and Nursery Characteristics of Several Citrus Rootstocks.几种柑橘砧木对柠檬疫霉和寄生疫霉的抗性及苗圃特性
Plant Dis. 1998 Nov;82(11):1217-1225. doi: 10.1094/PDIS.1998.82.11.1217.
8
Wide-ranging transcriptomic analysis of Poncirus trifoliata, Citrus sunki, Citrus sinensis and contrasting hybrids reveals HLB tolerance mechanisms.宽范围的枳、温州蜜柑、甜橙和杂交品种的转录组分析揭示了黄龙病抗性机制。
Sci Rep. 2020 Nov 30;10(1):20865. doi: 10.1038/s41598-020-77840-2.
9
Metabolic variations in different citrus rootstock cultivars associated with different responses to Huanglongbing.不同柑橘砧木品种的代谢变化与对黄龙病的不同反应相关。
Plant Physiol Biochem. 2016 Oct;107:33-44. doi: 10.1016/j.plaphy.2016.05.030. Epub 2016 May 20.
10
Physiologic, Anatomic, and Gene Expression Changes in Citrus sunki, Poncirus trifoliata, and Their Hybrids After 'Candidatus Liberibacter asiaticus' Infection.感染‘亚洲韧皮杆菌’后，四季橘、枳及其杂种的生理、解剖和基因表达变化
Phytopathology. 2017 May;107(5):590-599. doi: 10.1094/PHYTO-02-16-0077-R. Epub 2017 Mar 24.

本文引用的文献

1
Function, Discovery, and Exploitation of Plant Pattern Recognition Receptors for Broad-Spectrum Disease Resistance.植物模式识别受体的功能、发现和广谱抗病性的利用。
Annu Rev Phytopathol. 2017 Aug 4;55:257-286. doi: 10.1146/annurev-phyto-080614-120106. Epub 2017 Jun 15.
2
Effectors of Filamentous Plant Pathogens: Commonalities amid Diversity.丝状植物病原体的效应蛋白：多样性中的共性
Microbiol Mol Biol Rev. 2017 Mar 29;81(2). doi: 10.1128/MMBR.00066-16. Print 2017 Jun.
3
Potential of Burkholderia seminalis TC3.4.2R3 as Biocontrol Agent Against Fusarium oxysporum Evaluated by Mass Spectrometry Imaging.
利用质谱成像技术评价生防菌拜赖青霉 TC3.4.2R3 对尖孢镰刀菌的生防潜力。
J Am Soc Mass Spectrom. 2017 May;28(5):901-907. doi: 10.1007/s13361-017-1610-6. Epub 2017 Feb 13.
4
Fungal pathogenesis: Host modulation every which way.真菌致病机制：全方位的宿主调节。
Nat Microbiol. 2016 May 26;1(6):16075. doi: 10.1038/nmicrobiol.2016.75.
5
Metabolomic profiling of Burkholderia pseudomallei using UHPLC-ESI-Q-TOF-MS reveals specific biomarkers including 4-methyl-5-thiazoleethanol and unique thiamine degradation pathway.使用超高效液相色谱-电喷雾电离-四极杆-飞行时间质谱法对类鼻疽伯克霍尔德菌进行代谢组学分析，揭示了包括4-甲基-5-噻唑乙醇在内的特定生物标志物以及独特的硫胺素降解途径。
Cell Biosci. 2015 Jun 2;5:26. doi: 10.1186/s13578-015-0018-x. eCollection 2015.
6
An accelerated workflow for untargeted metabolomics using the METLIN database.使用METLIN数据库的非靶向代谢组学加速工作流程。
Nat Biotechnol. 2012 Sep;30(9):826-8. doi: 10.1038/nbt.2348.
7
Interkingdom metabolic transformations captured by microbial imaging mass spectrometry.微生物成像质谱捕获的跨王国代谢转化。
Proc Natl Acad Sci U S A. 2012 Aug 21;109(34):13811-6. doi: 10.1073/pnas.1206855109. Epub 2012 Aug 6.
8
Primer on agar-based microbial imaging mass spectrometry.琼脂基微生物成像质谱基础教程。
J Bacteriol. 2012 Nov;194(22):6023-8. doi: 10.1128/JB.00823-12. Epub 2012 Jul 20.
9
Observing the invisible through imaging mass spectrometry, a window into the metabolic exchange patterns of microbes.通过成像质谱观察不可见物，洞察微生物的代谢交换模式。
J Proteomics. 2012 Aug 30;75(16):5069-5076. doi: 10.1016/j.jprot.2012.05.036. Epub 2012 May 26.
10
MALDI-TOF MS in microbiological diagnostics-identification of microorganisms and beyond (mini review).基质辅助激光解吸电离飞行时间质谱技术在微生物诊断中的应用——微生物鉴定及其他（综述）。
Appl Microbiol Biotechnol. 2012 Feb;93(3):965-74. doi: 10.1007/s00253-011-3783-4. Epub 2011 Dec 25.