Son Yongwoo, Jin Yeung Bae, Cho Eun-Jeong, Park Ae Ra, Flores Rochelle A, Nguyen Binh T, Lee Seung Yun, Altanzul Bujinlkham, Park Kwang Il, Min Wongi, Kim Woo H
College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea.
Gyeongnam Veterinary Service Laboratory, Jinju 52733, Republic of Korea.
Vet Sci. 2024 Apr 16;11(4):180. doi: 10.3390/vetsci11040180.
The spread of antibiotic-resistant in the poultry industry poses significant public health challenges due to multidrug resistance and biofilm formation. We investigated the antibiotic resistance profiles and biofilm characteristics of and isolates from chicken meat in poultry slaughterhouses in South Korea. Ninety-six isolates (forty-eight each of and ) were collected between March and September 2022. Both species were analyzed using MALDI-TOF, PCR, antibiotic susceptibility testing, and biofilm assays. A high level of multidrug resistance was observed in (95.8%) and (93.8%), with exhibiting a broader range of resistance, particularly to linezolid (52.1%) and rifampicin (47.9%). All isolates formed biofilm in vitro, showing stronger biofilm formation than with a significant difference ( < 0.001) in biofilm strength. Specific genes (, , and ) were found to be correlated with biofilm strength. In isolates, biofilm strength was correlated with resistance to linezolid and rifampicin, while a general correlation between antibiotic resistance and biofilm strength was not established. Through analysis, correlations were noted between antibiotics within the same class, while no general trends were evident in other analyzed factors. This study highlights the public health risks posed by multidrug-resistant enterococci collected from poultry slaughterhouses, emphasizing the complexity of the biofilm-resistance relationship and the need for enhanced control measures.
家禽行业中抗生素耐药性的传播因多重耐药性和生物膜形成而对公共卫生构成重大挑战。我们调查了韩国家禽屠宰场鸡肉中肠球菌和屎肠球菌分离株的抗生素耐药谱及生物膜特征。2022年3月至9月间收集了96株分离株（肠球菌和屎肠球菌各48株）。对这两种菌均使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）、聚合酶链反应（PCR）、抗生素敏感性测试和生物膜检测进行分析。观察到肠球菌（95.8%）和屎肠球菌（93.8%）具有高度多重耐药性，肠球菌表现出更广泛的耐药范围，尤其是对利奈唑胺（52.1%）和利福平（47.9%）。所有肠球菌分离株在体外均形成生物膜，其生物膜形成能力强于屎肠球菌，生物膜强度存在显著差异（P<0.001）。发现特定基因（icaA、icaD和ebpS）与生物膜强度相关。在肠球菌分离株中，生物膜强度与对利奈唑胺和利福平的耐药性相关，而抗生素耐药性与生物膜强度之间未建立普遍相关性。通过分析，注意到同一类抗生素之间存在相关性，而在其他分析因素中未发现明显的总体趋势。本研究强调了从家禽屠宰场收集的多重耐药肠球菌对公共卫生的风险，强调了生物膜-耐药性关系的复杂性以及加强控制措施的必要性。
