Chen Chia-Yu, Chan Wai-Yin, Ismail Arshad, Oliver Shüné V
Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa.
Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, Division of the National Health Laboratory Service, Johannesburg 2193, South Africa.
Trop Med Infect Dis. 2024 Apr 13;9(4):84. doi: 10.3390/tropicalmed9040084.
The mosquito microbiota is a critical determinant of mosquito life history. It is therefore a target for novel vector control strategies like paratransgenesis. However, the microbiota in , a major African malaria vector, is poorly characterized. Thus, the study aimed to investigate the overall bacterial landscape in the salivary glands, ovaries and midguts of three laboratory strains of differing in insecticide-resistant phenotype by sequencing the V3-V4 hypervariable region of bacterial 16S rRNA genes. When examining alpha diversity, the salivary glands harbored significantly more bacteria in terms of species richness and evenness compared to ovaries and midguts. On the strain level, the insecticide-susceptible FANG strain had significantly lower bacterial diversity than the insecticide-resistant FUMOZ and FUMOZ-R strains. When looking at beta diversity, the compositions of microbiota between the three tissues as well as between the strains were statistically different. While there were common bacteria across all three tissues and strains of interest, each tissue and strain did exhibit differentially abundant bacterial genera. However, overall, the top five most abundant genera across all tissues and strains were , , , and . The presence of shared microbiota suggests a core microbiota that could be exploited for paratransgenesis efforts.
蚊子的微生物群是蚊子生活史的关键决定因素。因此，它是诸如共生转基因等新型病媒控制策略的目标。然而，非洲主要疟疾媒介冈比亚按蚊的微生物群特征尚不明确。因此，本研究旨在通过对细菌16S rRNA基因的V3 - V4高变区进行测序，调查三种具有不同抗杀虫剂表型的冈比亚按蚊实验室品系的唾液腺、卵巢和中肠中的整体细菌情况。在检查α多样性时，与卵巢和中肠相比，唾液腺在物种丰富度和均匀度方面含有更多的细菌。在品系水平上，杀虫剂敏感的FANG品系的细菌多样性显著低于抗杀虫剂的FUMOZ和FUMOZ - R品系。在查看β多样性时，三个组织之间以及品系之间的微生物群组成在统计学上存在差异。虽然在所有三个感兴趣的组织和品系中都有常见细菌，但每个组织和品系确实表现出不同丰度的细菌属。然而，总体而言，所有组织和品系中最丰富的前五个属是不动杆菌属、贪铜菌属、短波单胞菌属、假单胞菌属和伯克霍尔德菌属。共享微生物群的存在表明存在一个核心微生物群，可用于共生转基因研究。
