School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, Shaanxi, China.
Research Center of Basic Medical Science, Medical College, Qinghai University, Xining, 810016, Qinghai, China.
Environ Sci Pollut Res Int. 2024 May;31(21):31646-31655. doi: 10.1007/s11356-024-33472-x. Epub 2024 Apr 26.
The diversity of microalgae and bacteria allows them to form beneficial consortia for efficient wastewater treatment and nutrient recovery. This study aimed to evaluate the feasibility of a new microalgal-bacterial combination in the treatment of ice cream wastewater for biomass harvest. The bacterium Novosphingobium sp. ICW1 was natively isolated from ice cream wastewater and the microalga Vischeria sp. WL1 was a terrestrial oil-producing strain of Eustigmatophyceae. The ice cream wastewater was diluted 4 folds for co-cultivation, which was relatively less inhibitory for the growth of Vischeria sp. WL1. Four initial algal-bacterial combinations (v:v) of 150:0 (single algal cultivation), 150:1, 150:2, and 150:4 were assessed. During 24 days of co-cultivation, algal pigmentation was dynamically changed, particularly at the algal-bacterial combination of 150:4. Algal growth (in terms of cell number) was slightly promoted during the late phase of co-cultivation at the combinations of 150:2 and 150:4, while in the former the cellular oil yield was obviously elevated. Treated by these algal-bacterial combinations, total carbon was reduced by 67.5 ~ 74.5% and chemical oxygen demand was reduced by 55.0 ~ 60.4%. Although single bacterial treatment was still effective for removing organic nutrients, the removal efficiency was obviously enhanced at the algal-bacterial combination of 150:4. In addition, the harvested oils contained 87.1 ~ 88.3% monounsaturated fatty acids. In general, this study enriches the biotechnological solutions for the sustainable treatment of organic matter-rich food wastewater.
微藻和细菌的多样性使它们能够形成有益的共生体，从而实现高效的废水处理和养分回收。本研究旨在评估一种新型微藻-细菌组合在处理冰淇淋废水以收获生物质方面的可行性。细菌 Novosphingobium sp. ICW1 是从冰淇淋废水中天然分离出来的，微藻 Vischeria sp. WL1 是一种陆生产油的黄藻门菌株。将冰淇淋废水稀释 4 倍进行共培养，这对 Vischeria sp. WL1 的生长抑制作用相对较小。评估了四种初始藻菌组合（v:v），即 150:0（单一藻类培养）、150:1、150:2 和 150:4。在 24 天的共培养期间，藻色素动态变化，特别是在藻菌组合为 150:4 时。在共培养后期，组合 150:2 和 150:4 中的藻生长（以细胞数计）略有促进，而在前一种情况下，细胞油产量明显升高。用这些藻菌组合处理后，总碳减少了 67.5%74.5%，化学需氧量减少了 55.0%60.4%。虽然单一细菌处理仍能有效去除有机养分，但在藻菌组合为 150:4 时，去除效率明显提高。此外，所收获的油中含有 87.1%~88.3%的单不饱和脂肪酸。总的来说，本研究丰富了可持续处理富含有机物的食品废水的生物技术解决方案。
