Co-innovation Center of Neuroregeneration, Key laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, 226001 Nantong, PR China.
Co-innovation Center of Neuroregeneration, Key laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, 226001 Nantong, PR China; NMPA Key Laboratory for Quality Evaluation of Medical Protective and Implant Devices, 450018 Zhengzhou, PR China.
Int J Biol Macromol. 2024 May;268(Pt 2):131827. doi: 10.1016/j.ijbiomac.2024.131827. Epub 2024 Apr 25.
Cell culturing is a cornerstone of tissue engineering, playing a crucial role in tissue regeneration, drug screening, and the study of disease mechanisms. Among various culturing techniques, 3D culture systems, particularly those utilizing suspended fiber scaffolds, offer a more physiologically relevant environment than traditional 2D monolayer cultures. These 3D scaffolds enhance cell growth, differentiation, and proliferation by mimicking the in vivo cellular milieu. This review focuses on the critical role of suspended fiber scaffolds in tissue engineering. We compare the effectiveness of 3D suspended fiber scaffolds with 2D culture systems, discussing their respective benefits and limitations in the context of tissue regeneration. Furthermore, we explore the preparation methods of suspended fiber scaffolds and their potential applications. The review concludes by considering future research directions for optimizing suspended fiber scaffolds to address specific challenges in tissue regeneration, underscoring their significant promise in advancing tissue engineering and regenerative medicine.
细胞培养是组织工程的基石，在组织再生、药物筛选和疾病机制研究中发挥着关键作用。在各种培养技术中，3D 培养系统，特别是那些利用悬浮纤维支架的系统，比传统的 2D 单层培养提供了更接近生理的环境。这些 3D 支架通过模拟体内细胞环境来促进细胞生长、分化和增殖。本综述重点介绍了悬浮纤维支架在组织工程中的关键作用。我们比较了 3D 悬浮纤维支架与 2D 培养系统的效果，讨论了它们在组织再生方面各自的优缺点。此外，我们还探讨了悬浮纤维支架的制备方法及其潜在应用。最后，本文考虑了优化悬浮纤维支架以应对组织再生中特定挑战的未来研究方向，强调了它们在推进组织工程和再生医学方面的重要应用前景。
