Zhou Qin, Wang Yun, Yue Liang, Ye Ailing, Xie Xiaofan, Zhang Meilan, Tian Yuan, Liu Yang, Turatsinze Andéole Niyongabo, Constantine Uwaremwe, Zhao Xia, Zhang Yubao, Wang Ruoyu
Gansu Gaolan Field Scientific Observation and Research Station for Agricultural Ecosystem, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, Gansu, China.
Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province, Lanzhou, 730000, China.
BMC Plant Biol. 2024 Apr 26;24(1):340. doi: 10.1186/s12870-024-05024-5.
Astragalus mongholicus is a medicinal plant that is known to decrease in quality in response to continuous cropping. However, the differences in the root-associated microbiome and root exudates in the rhizosphere soil that may lead to these decreases are barely under studies. We investigated the plant biomass production, root-associated microbiota, and root exudates of A. mongholicus grown in two different fields: virgin soil (Field I) and in a long-term continuous cropping field (Field II). Virgin soil is soil that has never been cultivated for A. mongholicus. Plant physiological measurements showed reduced fresh and dry weight of A. mongholicus under continuous cropping conditions (i.e. Field II). High-throughput sequencing of the fungal and bacterial communities revealed differences in fungal diversity between samples from the two fields, including enrichment of potentially pathogenic fungi in the roots of A. mongholicus grown in Field II. Metabolomic analysis yielded 20 compounds in A. mongholicus root exudates that differed in relative abundance between rhizosphere samples from the two fields. Four of these metabolites (2-aminophenol, quinic acid, tartaric acid, and maleamate) inhibited the growth of A. mongholicus, the soil-borne pathogen Fusarium oxysporum, or both. This comprehensive analysis enhances our understanding of the A. mongholicus microbiome, root exudates, and interactions between the two in response to continuous cropping. These results offer new information for future design of effective, economical approaches to achieving food security.
蒙古黄芪是一种药用植物，已知其质量会因连作而下降。然而，导致这些质量下降的根际土壤中与根相关的微生物群和根分泌物的差异几乎未得到研究。我们调查了在两个不同田地中生长的蒙古黄芪的植物生物量生产、与根相关的微生物群和根分泌物：处女地（田地I）和长期连作田地（田地II）。处女地是从未种植过蒙古黄芪的土壤。植物生理测量表明，在连作条件下（即田地II）蒙古黄芪的鲜重和干重降低。对真菌和细菌群落的高通量测序揭示了来自两个田地的样本之间真菌多样性的差异，包括在田地II中生长的蒙古黄芪根中潜在致病真菌的富集。代谢组学分析在蒙古黄芪根分泌物中产生了20种化合物，这两种田地的根际样本中它们的相对丰度有所不同。其中四种代谢物（2-氨基酚、奎尼酸、酒石酸和马来酰胺）抑制了蒙古黄芪、土传病原菌尖孢镰刀菌的生长，或两者的生长。这种综合分析增强了我们对蒙古黄芪微生物群、根分泌物以及两者在连作响应中的相互作用的理解。这些结果为未来设计有效、经济的粮食安全保障方法提供了新信息。
