Genome-Wide Identification of the miRNA Family and Exploration of Their Expression Characteristics Caused by the Replant Disease Formation-Related Principal Factor.

, a highly valuable medicinal plant in China, is encountering severe replant disease. Replant disease represents a complex stress driven by multiple principal factors (RDFs), including allelochemicals, microbes, and their interactions. miRNAs are recognized as key regulators of plant response to stresses; however, their specific roles within RDFs are not entirely clear. This study builds six RDF treatments, comprising continuously planted (SP), normally planted (NP), and NP treated with ferulic acid (FA), (FO), and a combination of FA with FO (FAFO). sRNA-seq technology was used to identify crucial miRNAs in response to diverse RDFs. In total, 30 sRNA datasets were generated from the SP, NP, FA, FO, and FAFO samples. A total of 160 known and 41 novel miRNAs () were identified in the genome based on the sRNA database. Abundance analysis revealed that in SP exhibited a distinct expression profile in comparison with others. Of these, 124, 86, 86, and 90 were differentially expressed in SP, FA, FO, and FAFO compared with NP. Target analysis indicated that downregulated in both SP and RDFs impede the organism growth of . upregulated in SP can disrupt root formation and nutrient metabolism, in which, two were uniquely expressed in SP. It was confirmed to target genes. The expression patterns of and indicated that replant disease induces the oxidative damage of through . profiling under RDFs provides a theoretical basis for the further clarification of function in replant disease.