Ríos Susana, González Lina Gabriela, Saez Claudia Gilda, Smith Patricio Cristian, Escobar Lina M, Martínez Constanza Eugenia
School of Dentistry, Faculty of Medicine, Pontificia Universidad Catolica de Chile, Santiago 8330024, Chile.
Faculty of Dentistry, Universidad Nacional de Colombia, Bogotá 111321, Colombia.
Biomedicines. 2024 Apr 16;12(4):874. doi: 10.3390/biomedicines12040874.
Leukocyte and Platelet-Rich Fibrin (L-PRF) is part of the second generation of platelet-concentrates. L-PRF derived from nonsmokers has been used in surgical procedures, with its beneficial effects in wound healing being proven to stimulate biological activities such as cell proliferation, angiogenesis, and differentiation. Cigarette smoking exerts detrimental effects on tissue healing and is associated with post-surgical complications; however, evidence about the biological effects of L-PRF derived from smokers is limited. This study evaluated the impact of L-PRF secretome (LPRFS) derived from smokers and nonsmokers on angiogenesis and osteoblast differentiation. LPRFS was obtained by submerging L-PRF membranes derived from smokers or nonsmokers in culture media and was used to treat endothelial cells (HUVEC) or SaOs-2 cells. Angiogenesis was evaluated by tubule formation assay, while osteoblast differentiation was observed by alkaline phosphatase and osterix protein levels, as well as in vitro mineralization. LPRFS treatments increased angiogenesis, alkaline phosphatase, and osterix levels. Treatment with 50% of LPRFS derived from smokers and nonsmokers in the presence of osteogenic factors stimulates in vitro mineralization significantly. Nevertheless, differences between LPRFS derived from smokers and nonsmokers were not found. Both LPRFS stimulated angiogenesis and osteoblast differentiation in vitro; however, clinical studies are required to determine the beneficial effect of LPRFS in smokers.
富含白细胞和血小板的纤维蛋白（L-PRF）是第二代血小板浓缩物的一部分。来自不吸烟者的L-PRF已被用于外科手术，其在伤口愈合中的有益作用已被证明可刺激细胞增殖、血管生成和分化等生物活性。吸烟对组织愈合有不利影响，并与术后并发症相关；然而，关于来自吸烟者的L-PRF的生物学效应的证据有限。本研究评估了来自吸烟者和不吸烟者的L-PRF分泌组（LPRFS）对血管生成和成骨细胞分化的影响。通过将来自吸烟者或不吸烟者的L-PRF膜浸入培养基中获得LPRFS，并用于处理内皮细胞（HUVEC）或SaOs-2细胞。通过小管形成试验评估血管生成，同时通过碱性磷酸酶和osterix蛋白水平以及体外矿化观察成骨细胞分化。LPRFS处理增加了血管生成、碱性磷酸酶和osterix水平。在成骨因子存在的情况下，用50%来自吸烟者和不吸烟者的LPRFS处理可显著刺激体外矿化。然而，未发现来自吸烟者和不吸烟者的LPRFS之间存在差异。两种LPRFS均在体外刺激血管生成和成骨细胞分化；然而，需要进行临床研究以确定LPRFS对吸烟者的有益作用。
