Shi Huiqin, Li Wei, Chen Hongyu, Meng Yao, Wu Huifang, Wang Jian, Shen Shuo
Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China.
Key Laboratory of Potato Breeding of Qinghai Province, Xining 810016, China.
Int J Mol Sci. 2024 Apr 18;25(8):4437. doi: 10.3390/ijms25084437.
The potato dry rot disease caused by spp. seriously reduces potato yield and threatens human health. However, potential biocontrol agents cannot guarantee the stability and activity of biocontrol. Here, 18 synthetic microbial communities of different scales were constructed, and the synthetic microbial communities with the best biocontrol effect on potato dry rot disease were screened through in vitro and in vivo experiments. The results show that the synthetic community composed of , , , , and has the best biocontrol activity. Metabolomics results show that interacts with other member strains to produce caproic acid and reduce the disease index to 38.01%. Furthermore, the mycelial growth inhibition after treatment with caproic acid was 77.54%, and flow cytometry analysis showed that the living conidia rate after treatment with caproic acid was 11.2%. This study provides potential value for the application of synthetic microbial communities in potatoes, as well as the interaction mechanisms between member strains of synthetic microbial communities.
由[具体菌种]引起的马铃薯干腐病严重降低了马铃薯产量并威胁人类健康。然而，潜在的生物防治剂无法保证生物防治的稳定性和活性。在此，构建了18个不同规模的合成微生物群落，并通过体外和体内实验筛选出对马铃薯干腐病具有最佳生物防治效果的合成微生物群落。结果表明，由[具体菌种1]、[具体菌种2]、[具体菌种3]、[具体菌种4]、[具体菌种5]和[具体菌种6]组成的合成群落具有最佳的生物防治活性。代谢组学结果表明，[具体菌种]与其他成员菌株相互作用产生己酸，并将病情指数降低至38.01%。此外，己酸处理后的菌丝生长抑制率为77.54%，流式细胞术分析表明，己酸处理后的活分生孢子率为11.2%。本研究为合成微生物群落在马铃薯中的应用以及合成微生物群落成员菌株之间的相互作用机制提供了潜在价值。
