Li Chenglong, Han Yanfeng, Zou Xiao, Zhang Xueqian, Ran Qingsong, Dong Chunbo
Institute of Fungus Resources, Department of Ecology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, China.
Synth Syst Biotechnol. 2024 Jun 20;9(4):775-783. doi: 10.1016/j.synbio.2024.06.006. eCollection 2024 Dec.
Synthetic microbial community has widely concerned in the fields of agriculture, food and environment over the past few years. However, there is little consensus on the method to synthetic microbial community from construction to functional verification. Here, we review the concept, characteristics, history and applications of synthetic microbial community, summarizing several methods for synthetic microbial community construction, such as isolation culture, core microbiome mining, automated design, and gene editing. In addition, we also systematically summarized the design concepts, technological thresholds, and applicable scenarios of various construction methods, and highlighted their advantages and limitations. Ultimately, this review provides four efficient, detailed, easy-to-understand and -follow steps for synthetic microbial community construction, with major implications for agricultural practices, food production, and environmental governance.
在过去几年中，合成微生物群落已在农业、食品和环境领域受到广泛关注。然而，从合成微生物群落的构建到功能验证，目前在方法上几乎没有达成共识。在此，我们综述了合成微生物群落的概念、特征、历史及应用，总结了几种合成微生物群落构建方法，如分离培养、核心微生物组挖掘、自动化设计和基因编辑。此外，我们还系统总结了各种构建方法的设计理念、技术门槛和适用场景，并突出了它们的优点和局限性。最终，本综述为合成微生物群落构建提供了四个高效、详细、易懂且可遵循的步骤，对农业实践、食品生产和环境治理具有重要意义。
