Čeprnja Toni, Tomić Snježana, Perić Balja Melita, Marušić Zlatko, Blažićević Valerija, Spagnoli Giulio Cesare, Juretić Antonio, Čapkun Vesna, Vuger Ana Tečić, Pogorelić Zenon, Mrklić Ivana
Department of Pathology, Forensic Medicine and Cytology, University Hospital of Split, 21000 Split, Croatia.
Department of Pathology, School of Medicine, University of Split, 21000 Split, Croatia.
Int J Mol Sci. 2024 Apr 20;25(8):4513. doi: 10.3390/ijms25084513.
"Basal-like" (BL) morphology and the expression of cancer testis antigens (CTA) in breast cancer still have unclear prognostic significance. The aim of our research was to explore correlations of the morphological characteristics and tumor microenvironment in triple-negative breast carcinomas (TNBCs) with multi-MAGE-A CTA expression and to determine their prognostic significance. Clinical records of breast cancer patients who underwent surgery between January 2017 and December 2018 in four major Croatian clinical centers were analyzed. A total of 97 non-metastatic TNBCs with available tissue samples and treatment information were identified. Cancer tissue sections were additionally stained with programmed death-ligand 1 (PD-L1) Ventana (SP142) and multi-MAGE-A (mAb 57B). BL morphology was detected in 47 (49%) TNBCs and was associated with a higher Ki-67 proliferation index and histologic grade. Expression of multi-MAGE-A was observed in 77 (79%) TNBCs and was significantly associated with BL morphology. Lymphocyte-predominant breast cancer (LPBC) status was detected in 11 cases (11.3%) and significantly correlated with the Ki-67 proliferation index, increased number of intratumoral lymphocytes (itTIL), and PD-L1 expression. No impact of BL morphology, multi-MAGE-A expression, histologic type, or LPBC status on disease-free survival was observed. Our data suggest that tumor morphology could help identify patients with potential benefits from CTA-targeting immunotherapy.
“基底样”（BL）形态以及癌胚抗原（CTA）在乳腺癌中的表达，其预后意义仍不明确。我们研究的目的是探讨三阴性乳腺癌（TNBC）的形态学特征和肿瘤微环境与多MAGE-A CTA表达之间的相关性，并确定它们的预后意义。对2017年1月至2018年12月期间在克罗地亚四个主要临床中心接受手术的乳腺癌患者的临床记录进行了分析。共识别出97例有可用组织样本和治疗信息的非转移性TNBC。癌组织切片另外用程序性死亡配体1（PD-L1）Ventana（SP142）和多MAGE-A（单克隆抗体57B）进行染色。在47例（49%）TNBC中检测到BL形态，并且与较高的Ki-67增殖指数和组织学分级相关。在77例（79%）TNBC中观察到多MAGE-A的表达，并且与BL形态显著相关。在11例（11.3%）中检测到淋巴细胞为主型乳腺癌（LPBC）状态，并且与Ki-67增殖指数、瘤内淋巴细胞（itTIL）数量增加和PD-L1表达显著相关。未观察到BL形态、多MAGE-A表达、组织学类型或LPBC状态对无病生存期的影响。我们的数据表明，肿瘤形态学有助于识别可能从CTA靶向免疫治疗中获益的患者。
