Ai Jiaqi, Wang Wuhong, Hu Tianhua, Hu Haijiao, Wang Jinglei, Yan Yaqin, Pang Hongtao, Wang Yong, Bao Chonglai, Wei Qingzhen
Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou 310021, China.
Genes (Basel). 2024 Mar 26;15(4):415. doi: 10.3390/genes15040415.
Seed dormancy is a life adaptation trait exhibited by plants in response to environmental changes during their growth and development. The dormancy of commercial seeds is the key factor affecting seed quality. Eggplant seed dormancy is controlled by quantitative trait loci (QTLs), but reliable QTLs related to eggplant dormancy are still lacking. In this study, F populations obtained through the hybridization of paternally inbred lines with significant differences in dormancy were used to detect regulatory sites of dormancy in eggplant seeds. Three QTLs (, , and ) related to seed dormancy were detected on three chromosomes of eggplant using the QTL-Seq technique. By combining nonsynonymous sites within the candidate regions and gene functional annotation analysis, nine candidate genes were selected from three QTL candidate regions. According to the germination results on the eighth day, the male parent was not dormant, but the female parent was dormant. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the expression of nine candidate genes, and the gene showed roughly the same trend as that in the phenotypic data. We proposed as the potential key gene involved in regulating the dormancy of eggplant seeds. The results of seed experiments with different concentrations of gibberellin A (GA) showed that, within a certain range, the higher the gibberellin concentration, the earlier the emergence and the higher the germination rate. However, higher concentrations of GA may have potential effects on eggplant seedlings. We suggest the use of GA at a concentration of 200-250 mg·L to treat dormant seeds. This study provides a foundation for the further exploration of genes related to the regulation of seed dormancy and the elucidation of the molecular mechanism of eggplant seed dormancy and germination.
种子休眠是植物在生长发育过程中为响应环境变化而表现出的一种生活适应特性。商品种子的休眠是影响种子质量的关键因素。茄子种子休眠受数量性状位点（QTL）控制，但仍缺乏与茄子休眠相关的可靠QTL。本研究利用休眠性差异显著的父本自交系杂交获得的F群体来检测茄子种子休眠的调控位点。采用QTL-Seq技术在茄子的三条染色体上检测到3个与种子休眠相关的QTL（、和）。通过结合候选区域内的非同义位点和基因功能注释分析，从3个QTL候选区域中筛选出9个候选基因。根据第8天的萌发结果，父本不休眠，而母本休眠。采用实时荧光定量聚合酶链反应（qRT-PCR）对9个候选基因的表达进行验证，基因的表达趋势与表型数据大致相同。我们提出作为调控茄子种子休眠的潜在关键基因。不同浓度赤霉素A（GA）种子试验结果表明，在一定范围内，赤霉素浓度越高，出苗越早，发芽率越高。然而，较高浓度的GA可能对茄子幼苗有潜在影响。我们建议使用浓度为200-250mg·L的GA处理休眠种子。本研究为进一步探索种子休眠调控相关基因及阐明茄子种子休眠和萌发的分子机制奠定了基础。
