Department of Biochemistry, Vivekanandha College of Arts and Sciences for Women (Autonomous), Elayampalayam, Tiruchengode, Namakkal, Tamil Nadu, 637205, India.
Department of Biochemistry, K.S.Rangasamy College of Arts and Science, Tiruchengode, Namakkal, Tamil Nadu, 637215, India.
Biotechnol Lett. 2024 Oct;46(5):871-885. doi: 10.1007/s10529-024-03491-2. Epub 2024 Apr 27.
Nanocomposites selectively induce cancer cell death, holding potential for precise liver cancer treatment breakthroughs. This study assessed the cytotoxicity of gold nanocomposites (Au NCs) enclosed within silk fibroin (SF), aptamer (Ap), and the myogenic Talaromyces purpureogenus (TP) against a human liver cancer cell (HepG2). The ultimate product, Ap-SF-TP@Au NCs, results from a three-step process. This process involves the myogenic synthesis of TP@Au NCs derived from TP mycelial extract, encapsulation of SF on TP@Au NCs (SF-TP@Au NCs), and the conjugation of Ap within SF-TP@Au NCs. The synthesized NCs are analyzed by various characteristic techniques. Ap-SF-TP@Au NCs induced potential cell death in HepG2 cells but exhibited no cytotoxicity in non-cancerous cells (NIH3T3). The morphological changes in cells were examined through various biochemical staining methods. Thus, Ap-SF-TP@Au NCs emerge as a promising nanocomposite for treating diverse cancer cells.
纳米复合材料选择性诱导癌细胞死亡，为精确治疗肝癌提供了潜在突破。本研究评估了金纳米复合材料（Au NCs）封装在丝素蛋白（SF）、适配体（Ap）和肌肉生成的紫色红曲霉（TP）中的细胞毒性，针对人肝癌细胞（HepG2）。最终产物 Ap-SF-TP@Au NCs 是通过三步过程产生的。该过程涉及从 TP 菌丝体提取物中合成的 TP@Au NCs 的肌生成、SF 在 TP@Au NCs 上的封装（SF-TP@Au NCs）以及 Ap 在 SF-TP@Au NCs 内的缀合。通过各种特征技术分析合成的 NCs。Ap-SF-TP@Au NCs 诱导 HepG2 细胞发生潜在的细胞死亡，但对非癌细胞（NIH3T3）没有细胞毒性。通过各种生化染色方法检查细胞的形态变化。因此，Ap-SF-TP@Au NCs 是一种很有前途的用于治疗各种癌细胞的纳米复合材料。
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