基于光子晶体信号放大的自校准比率荧光传感器用于食品中环丙沙星的检测。
A self-calibrating ratiometric fluorescence sensor with photonic crystal-based signal amplification for the detection of tetracycline in food.
机构信息
College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China..
College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China.
出版信息
Food Chem. 2024 Sep 1;451:139418. doi: 10.1016/j.foodchem.2024.139418. Epub 2024 Apr 22.
A dual-color ratiometric fluorescence sensor based on photonic crystals (PCs) was developed to detect tetracycline (TC) in food. PC was fabricated via self-assembly of carbon dots (CDs)-loaded SiO nanoparticles. Gold nanoclusters (AuNCs) and copper ions (Cu) were then adsorbed onto the PC for sensor fabrication. The fluorescence of AuNCs was amplified by the PC with an enhancement ratio of 7.6, providing higher sensitivity. The fluorescence of AuNCs was quenched by Cu, whereas that of CDs remained unchanged as an internal reference. TC restored the fluorescence of AuNCs owing to its complexation with Cu, resulting in a change in the fluorescence intensity ratio. The sensor exhibited a good linear relationship with TC concentrations ranging from 0.1 to 10 μM, with a detection limit of 34 nM. Furthermore, the sensor was applied for TC detection in food with satisfactory recoveries and relative standard deviations, revealing great potential in practical application.
一种基于光子晶体（PCs）的双色比率荧光传感器被开发出来，用于检测食品中的四环素（TC）。PC 通过负载碳点（CDs）的 SiO 纳米粒子自组装而成。然后将金纳米团簇（AuNCs）和铜离子（Cu）吸附到 PC 上用于传感器的制备。AuNCs 的荧光通过增强比为 7.6 的 PC 得到放大，从而提供更高的灵敏度。Cu 猝灭了 AuNCs 的荧光，而 CDs 的荧光则保持不变作为内部参考。由于与 Cu 的络合作用，TC 恢复了 AuNCs 的荧光，导致荧光强度比发生变化。该传感器在 0.1 至 10 μM 的 TC 浓度范围内表现出良好的线性关系，检测限为 34 nM。此外，该传感器还应用于食品中 TC 的检测，具有令人满意的回收率和相对标准偏差，显示出在实际应用中的巨大潜力。
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