Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Cell Mol Biol (Noisy-le-grand). 2024 Apr 28;70(4):8-14. doi: 10.14715/cmb/2024.70.4.2.
The existence of diverse microbes in unprocessed camel milk poses a significant threat to the well-being of a large population, especially infants and toddlers. The objective of this study was to ascertain the existence of microorganisms in unprocessed raw camel milk by employing a molecular-based technique in combination with a histological examination of bacteria. The identification of microbial species was achieved by employing PCR amplification and sequencing of 16s rRNA gene fragments. Various micorganisms found includes the probiotic Lactobacillus species, Staphylococcus succinic, Macrococcus casealyticus, Bacillus cohnii, and Salinicoccus kunmingensis. To prevent microbial contamination in raw milk, it is necessary to adequately heat or pasteurise the milk and to wash and sterilise the udder before milking the camel. This is because raw milk contains microbes that cause multiple diseases. Moreover, in the current era of the COVID-19 pandemics, ensuring proper sanitary conditions in milk and its derivatives might potentially mitigate the transmission of various diseases among consumers shortly. Keywords: camel, microbiota, 16s rRNA gene, PCR.
未加工骆驼奶中存在多种微生物,对大量人群,尤其是婴儿和幼儿的健康构成重大威胁。本研究的目的是通过分子技术结合细菌组织学检查,确定未加工生骆驼奶中微生物的存在。通过对 16s rRNA 基因片段进行 PCR 扩增和测序来鉴定微生物种类。发现的各种微生物包括益生菌乳杆菌属、琥珀酸葡萄球菌、巨球菌属、柯恩氏芽孢杆菌和昆明盐球菌。为了防止生奶中的微生物污染,有必要充分加热或巴氏杀菌,并在挤奶前清洗和消毒乳房。这是因为生奶中含有多种致病微生物。此外,在当前 COVID-19 大流行的时代,确保奶及其制品的适当卫生条件可能会在短期内降低消费者之间各种疾病的传播风险。
骆驼、微生物组、16s rRNA 基因、PCR。