Ma Wanpeng, Zhang Lin, Yao Huaibing, Zhang Yi, Wang Wei, Liu Yifan, Zhao Xueting, Su Zhanqiang
College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
Xinjiang Key Laboratory of New Drug Research and Development for Herbivorous Animals, Urumqi 830052, China.
Microorganisms. 2025 Jun 15;13(6):1394. doi: 10.3390/microorganisms13061394.
Subclinical mastitis is a critical disease affecting camel health and milk quality. However, research on shifts in milk bacterial communities following subclinical mastitis in camels is limited. We evaluated changes in bacterial communities following subclinical mastitis in Bactrian camels. Three portions of California Mastitis Test (CMT)-negative milk and five portions of CMT-positive milk were collected from each Jimunai County and Keping County using the CMT, and the bacterial community composition of the camel milk was analyzed using amplicon sequencing of the v34 region of the 16S rRNA gene. Subclinical mastitis induced genus-level differences in the core bacterial microbiota of Bactrian camel milk. To our knowledge, was identified in camel milk for the first time, predominantly in Jimunai County. Bacterial abundance in camel milk from Keping County was increased and altered. Alpha diversity analysis revealed that subclinical mastitis induced lower and higher bacterial abundance in milk from Jimunai County and Keping County, respectively, compared to that of healthy camels. Therefore, these findings provide direction for future research on pathogenic microorganisms for the prevention and control of subclinical mastitis in Bactrian camels.
亚临床乳腺炎是一种影响骆驼健康和牛奶质量的关键疾病。然而，关于骆驼亚临床乳腺炎后牛奶细菌群落变化的研究有限。我们评估了双峰驼亚临床乳腺炎后细菌群落的变化。使用加利福尼亚乳腺炎检测（CMT）从吉木乃县和柯坪县的每头骆驼中采集三份CMT阴性牛奶和五份CMT阳性牛奶，并通过对16S rRNA基因v34区域的扩增子测序分析骆驼奶的细菌群落组成。亚临床乳腺炎导致双峰驼奶核心细菌微生物群在属水平上存在差异。据我们所知，首次在骆驼奶中鉴定出[具体内容缺失]，主要在吉木乃县。柯坪县骆驼奶中的细菌丰度增加且发生了改变。α多样性分析表明，与健康骆驼相比，亚临床乳腺炎分别导致吉木乃县和柯坪县牛奶中细菌丰度降低和升高。因此，这些发现为未来双峰驼亚临床乳腺炎防控病原微生物的研究提供了方向。
